Does anyone know these biology questions?
In order to follow the 3:1 insert to vector molar ratio for ligation, 0.06 pmol of the insert and 0.02 pmol of the vector is added to a microcentrifuge tube, along with T4 DNA ligase, appropriate buffer, and diH2O.
a. Calculate the volume of digested and BamHI-free pUC19 to add to the ligation reaction. Round to the nearest one-tenth in microliter. Include units.
b. The insert DNA, which contains the inlA gene, is 2410 bp. After digesting with BamHI and cleaned using the in silica column, the new concentration was found to be 10 ng/μl. Calculate the volume of digested and BamHI-free insert DNA to add to the ligation reaction. Round to the nearest one-tenth in microliter. Include units.
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See image please show full solution, (Indicate your answers to two digits after the decimal) 8....
See image
please show full solution,
(Indicate your answers to two
digits after the decimal)
8. You have two plasmid preparations "A and "B" (illustrated below) each at concentrations of 100nguL. You wish to subclone a 1.5 Kbp EcoRI fragment from plasmid·A" into the unique EcoRI site of a 3.0Kbp vector"B". EcoRI 1.5 Kbp Total size 3.0Kbp BT Total size 7.5Kbp Complete the following table to indicate the volumes of each ingredient required for the preparation of a 50μL ligation...
I just need the answers to questions 2 and 3. My DNA ladder is in lane...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
very lost on this whole worksheet and i dont know if my current answers are even...
very lost on this whole worksheet and i dont know if my
current answers are even right, please help i need this done by
today
Name: Cloning Worksheet chromosome qut 756bp pBiochem 5.983 bp ladder Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 BamHI (4233) (230) IT Enzyme recognition S ..GATATC. 51 EcoRV S .. . 3..CTATAC... 5 12 Pvull 89 BamHI Pett You are given the task to clone yfg into the plasmid pBioChem. Follow...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
See image
please show full solution,
(Indicate your answers to two
digits after the decimal)
8. You have two plasmid preparations "A and "B" (illustrated below) each at concentrations of 100nguL. You wish to subclone a 1.5 Kbp EcoRI fragment from plasmid·A" into the unique EcoRI site of a 3.0Kbp vector"B". EcoRI 1.5 Kbp Total size 3.0Kbp BT Total size 7.5Kbp Complete the following table to indicate the volumes of each ingredient required for the preparation of a 50μL ligation...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
very lost on this whole worksheet and i dont know if my
current answers are even right, please help i need this done by
today
Name: Cloning Worksheet chromosome qut 756bp pBiochem 5.983 bp ladder Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 BamHI (4233) (230) IT Enzyme recognition S ..GATATC. 51 EcoRV S .. . 3..CTATAC... 5 12 Pvull 89 BamHI Pett You are given the task to clone yfg into the plasmid pBioChem. Follow...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
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