Homework Answers
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Too little bacterial inoculum will give a bigger area of zone effect, which may be the whole plate. It is because not all the amount of the antibiotic or bactericide could be utilized. This will not provide a sufficient effect of the chemical on an optimum bacterial concentration.
Too heavy inoculum will grow more amount of bacteria on the plate, this time the antibiotic or bactericide may or may not able to show the zone of inhibition. It is because too much concentration of bacterial cells will hide the effect of the chemical.
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4) What zone effects would you get by putting too heavy or light amount of bacterial...
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1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
ECO 2013. Imagine that you are famous for putting on the most incredible firework and light...
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2. In what units of measure do we use for a zone of inhibition? 3. Given...
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What (other than motility) bacterial structural (as seen with a light microscope) or biochemical characteristics could...
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12a. If you plated transformed cells on LB, what would the growth look like? Why? b....
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Lab Exercise - Ultraviolet Light-Lethal Effects Please review the below videos as well as read introduction, materials, methods & procedures in the lab manuals and answer the following questions. https://www.youtube.com/watch?v=z4arnMlhbpE&app=desktop https://www.youtube.com/watch?v=OMN9ZGSHZW 1) Why was half of each plate covered with an index card? 2) Suppose you've tested the effect of UV radiation on bacterial growth for E. coli vs. S. aureus plates. How did the growth on E. Coli plates differ from the growth on the S. aureus plates that...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
2. In what units of measure do we use for a zone of inhibition? 3. Given the picture shown here, what steps do you need to go through to decide which of these antibiotics would be best for use on a patient? Zone with no bacterial growth Bacteria growing on agar gel Antibiotic disc 4. What was the purpose of the catalase test that you did for the Unknown Identification? 5. Explain how each of the following contributes to bacterial...
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