1. how do you determine the culture amount needed to prepare a bacterial smear? 2. what would be ...
1. how do you determine the culture amount needed to
prepare a bacterial smear?
2. what would be possible outcomes of a staining done in a slide
with high cell concentrations?
3. what would be he problem if your smear has a low cell
concentration?
Homework Answers
1. Smears typically require only a small amount of bacterial culture. In case of bacterial suspensions in broth a loopful of the broth culture on the slide is sufficient. In case of bacterial plate cultures a very small sample of a bacterial colony is taken by the loop and gently stir into the drop of water/saline on the slide to create an emulsion.
2. Slide with high cell concentrations will form a very thick smear which diminishes the amount of light that can pass through, thus making it difficult to visualize the morphology of single cells. Also, there is possibility of irregular staining.
3. Low cell concentration in a smear can result in a smear where bacteria will be too difficult to find.
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