Question

. You are given the following time course data: (Control tube differs from experimental tube in that it does not contain enzyme.) Control Time (minutes) ABS @405nm Experimental ABS 405nm 0.001 0.060 0.103 0.220 0.350 0 20 30 40 0.002 0.150 0.321 0.520 0.750 What can you determine from your analysis of this data? (5 points) . nswer #3a after this line Provide two possible explanations for the differences observed in the absorbance values of the experimental” tube and “control tube. (5 points)
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Answer #1

A) From this data we can determine that the conversion of product from substrate rate is increased by the enzyme but this reaction doesn't strictly needs enzyme because the absorbance in the control tube is also increasing.

B) There are differences in the results of control and experimental tubes the experimental tubes contain enzyme and control tube doesn't that is why there is more absorbance and second is as enzymes are present in the experimental tube more products are produced than the control tube and that increase the absorbance in the experimental tube.

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