Question

Suppose you are trying to synthesize DNA molecules In test tubes in the laboratory. A single stranded DNA molecule with the sequence 3'- GCGCGGAGTCCCTATCTGCA-5' will be used as the template strand for DNA synthesis. You also have at your disposal an DNA primer with the sequence 5'- CGCGCC-3', a DNA polymerase, and nucleotides You perform Iive separate experiments using the nucleotides indicated below: Experiment 1: dATP, dCTP, dGTP, and dTTP Experiment 2 dATP, dCTP. GTP, and dTTP Experiment 3 dATP, dCMP, dGTP, and dTTP Experiment 4: dATP, ddCTP, dGTP, and UTP Experiment 5 dATP, dCTP, dGDP, and ddTTP What DNA sequence will be produced in each of the five attempts at DNA synthesis? For this question, assume that when the DNA polymerase encounters a base that it cannot incorporate, DNA synthesis is terminated prematurely alter the last incorporated nucleotide. Please do not include the sequence of the primer in your answers.

Answer 1

1) TCAGGGATAGACGT

2) TCA

3) A

4)UCAGGGAUAGACGU

5) TCA

As chain gets terminated as it encounters the wrong nucleotide so these are sequence which gets incorporated