Homework Answers
The relative mobility of a protein on SDS-PAGE is dependent on its size and molecular weight.
If molecular weight of a protein is very higher than the other proteins in the loaded wells, then it's relative mobility will be very lesser than the remaining proteins in the SDS PAGE.
From the given scatter plot, the circled protein's log value of its molecular weight is 4.7.
Then the molecular weight of circular protein is 50,118.8 daltons, because the log value of 50,118.8 is 4.7.
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The log of the molecular weights of proteins can be plotted against the relative mobility on...
Predict the patterns (number of bands and apparent molecular weights) of the following proteins on SDS...
Predict the patterns (number of bands and apparent molecular weights) of the following proteins on SDS gels: a. A monomeric protein with a molecular weight of 35,000 Da b. A trimetric protein containing three chains, each with a molecular weight of 60,000 Da c. Immunoglobulin G (Nelson and Cox, page 178) in a non-reducing gel (no beta -mercaptoethanol added in the sample solution) - the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with me is found in rabbit serum. This protein is called serumbumin. The sandard unknown proteins and the rabbit serum prins for this experimentarehe pre- tined to allow you to follow the portion during crophews Background Information A first step in the analysis of a protein in the molecular biology laboratory frequently involves determination of its sie or molecular weight. The molecular weight of a...
this is whst i have so its mire spread out. im not sure what else to...
this is whst i have so its mire spread out. im not sure what
else to add.. whst should i be looking for?
koa AB Top of Resolvine 200 116 31 22 14 WellA: MW (Protein Ladder). Well B: Sample Protein 1. Well : Sample Protein 2. Dye front Activities: Based on the picture of the destained SDS-PAGE gel, complete the following Information: -Migration distance of dye front: mm. Molecular Weight Log (MW) Distance travelled Ri (Da) of protein (mm)...
Why are proteins heat denatured prior to analysis in SDS-PAGE? Select all answers that apply. Denaturation...
Why are proteins heat denatured prior to analysis in SDS-PAGE? Select all answers that apply. Denaturation of the protein is necessary so that proteins run proportionally to their size, based on the interaction of SDS with the unfolded protein. Heat is used to hydrolyze the peptide bonds of the protein. The heat step allows the proteins to unfold, enabling the protein chain to be coated with SDS molecules. Heat is used to hydrolyze disulfide bonds. QUESTION 2 1.5 points Saved...
0.5. What fraction of the offsprings of two parents with Sickle cell trait would you expect...
0.5. What fraction of the offsprings of two parents with Sickle cell trait would you expect to have Sickle cell anemia? Q.6. List the major steps you undertake for performing ELISA? 0.7. Describe an ELISA procedure you'll undertake to determine if a person is infected with AIDS virus? Q.3. In the two protein gel electrophoresis labs done in this class, proteins were separated based on their net charge or their size (weight). Explain similarities and differences between these two laboratory...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
identify the 25 correct statements in the set below identify 25 correct statements Please identify the...
identify the 25 correct statements in the set below
identify 25 correct statements
Please identify the 25 correct statements in the set below: Discovery analytics focuses on the question "Why did it happen?" Predicting a presidential candidate's percentage of the statewide vote from a sample of 800 voters would be an example of inferential statistics. This year, Oxnard University produced two football All-Americans. This is an example of continuous data. Sturges' Rule is merely a suggestion, not an ironclad requirement....
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
Predict the patterns (number of bands and apparent molecular weights) of the following proteins on SDS gels: a. A monomeric protein with a molecular weight of 35,000 Da b. A trimetric protein containing three chains, each with a molecular weight of 60,000 Da c. Immunoglobulin G (Nelson and Cox, page 178) in a non-reducing gel (no beta -mercaptoethanol added in the sample solution) - the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with me is found in rabbit serum. This protein is called serumbumin. The sandard unknown proteins and the rabbit serum prins for this experimentarehe pre- tined to allow you to follow the portion during crophews Background Information A first step in the analysis of a protein in the molecular biology laboratory frequently involves determination of its sie or molecular weight. The molecular weight of a...
this is whst i have so its mire spread out. im not sure what
else to add.. whst should i be looking for?
koa AB Top of Resolvine 200 116 31 22 14 WellA: MW (Protein Ladder). Well B: Sample Protein 1. Well : Sample Protein 2. Dye front Activities: Based on the picture of the destained SDS-PAGE gel, complete the following Information: -Migration distance of dye front: mm. Molecular Weight Log (MW) Distance travelled Ri (Da) of protein (mm)...
Why are proteins heat denatured prior to analysis in SDS-PAGE? Select all answers that apply. Denaturation of the protein is necessary so that proteins run proportionally to their size, based on the interaction of SDS with the unfolded protein. Heat is used to hydrolyze the peptide bonds of the protein. The heat step allows the proteins to unfold, enabling the protein chain to be coated with SDS molecules. Heat is used to hydrolyze disulfide bonds. QUESTION 2 1.5 points Saved...
0.5. What fraction of the offsprings of two parents with Sickle cell trait would you expect to have Sickle cell anemia? Q.6. List the major steps you undertake for performing ELISA? 0.7. Describe an ELISA procedure you'll undertake to determine if a person is infected with AIDS virus? Q.3. In the two protein gel electrophoresis labs done in this class, proteins were separated based on their net charge or their size (weight). Explain similarities and differences between these two laboratory...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
identify the 25 correct statements in the set below
identify 25 correct statements
Please identify the 25 correct statements in the set below: Discovery analytics focuses on the question "Why did it happen?" Predicting a presidential candidate's percentage of the statewide vote from a sample of 800 voters would be an example of inferential statistics. This year, Oxnard University produced two football All-Americans. This is an example of continuous data. Sturges' Rule is merely a suggestion, not an ironclad requirement....
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Most questions answered within 3 hours.
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