Question

Smol-L R mol L min) 10.0 20.0 The data in the table was collected for a certain enzyme-catalyzed reaction. Use these data to determine the maximum rate of the enzyme (Rmax), often called the maximum velocity (Vmax), and the Michaelis-Menten constant of the enzyme (KM). If you were to plot this data to graphically determine Rmax and Ky using a Lineweaver-Burk plot, what would you plot? If you would use the original data in the table.er original value for your answer. 500 100.0 2000 S 5.0 pmol L . Graph as 22 mol L "min":Graph as S] 20.0 mol-L 'Graph as Garut

Answer 1

Micheli's menten equation Ne Umane Cs] km + C5] here, where as amor . R = Rmax [s] RMt [s] * remote contre tren straight line egne y=m*tc comparing ① and ② Hence, to find kry and know, we need plot - 6 vs is IM ple mch

For, [S] value as 5 μmol L^-1, 1/[S] = 1/5 = 0.2

Graph as 0.2 on the x-axis.

For, R value is 22 μmol L^-1 min^-1, Hence 1/R = 1/22 = 0.045

Graph as 0.045 on the y- axis.

For [S] = 20 μmol L^-1,  1/[S] = 1/20 = 0.05

Graph as 0.05 on the x-axis.