Question

Make a comparative chart between the Western blot, Northern blot and Southern blot technique.

Method	Applications	Reagents	Process
Western blot
Southern blot
Northern blot

Answer 1

Comparative chart between the Western blot, Northern blot and Southern blot.

Method	Applications	Reagents	Process
Western blot	used to confirm a positive HIV diagnosis.	methanol, glycine,dried milk, stains like Amido black, Alcian blue and Ponceau S.	The process includes five steps namely transfer,blocking, primary antibody incubation, secondary antibody incubation and protein detection and analysis.
Southern blot	used to detect specific DNA sequence from DNA sample.	SDS, 6X SSC, 5X Denhardt's solution, yeast tRNA.	The process involves transfer of electrophoresis separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.
Northern blot	used to detect specific RNA molecules from a mixture of RNA.	EDTA, SDS, Salmon sperm DNA, sodium heparin etc.	The process includes separation of isolated RNA by gel electrophoresis, transferring to a membrane and detection of hybridization with a DNA or RNA probe.