what is the role of Tris.HCl buffer in carbonic anhydrase activity assay?
what is the role of Tris.HCl buffer in carbonic anhydrase activity assay?
Homework Answers
Tris-HCl buffer, 0.1. M (pH 8.3) allowed for a better stabilization of the enzyme during extraction and estimation.
Tris-HCl is buffer and hence it maintains the PH of the solution during the assay.
Hence carbonic anhydrase does to degrade or destroy and shows instant activity of this enzyme.
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what is the role of Tris.HCl buffer in carbonic anhydrase
activity assay?
You start thinking about the mechanism of carbonic anhydrase. Describe what role the metal(mercury) plays in...
You start thinking about the mechanism of carbonic anhydrase. Describe what role the metal(mercury) plays in the reaction and the other type of catalysis present in carbonic anhydrase. Mercury was found to be an inhibitor in a previous question.
15. Compare the catalytic activity of of the following enzymes: carbonic anhydrase, aspartate transcarbomylase (ATcase), restriction...
15. Compare the catalytic activity of of the following enzymes: carbonic anhydrase, aspartate transcarbomylase (ATcase), restriction enzymes and myosins. (40 points) What is unique about each active site with regards to types of amino acid residues a) located in active site and interaction with substrate? b) Briefly what is the mechanism of catalysis in each active site? Are cofactors or coenzymes required by the enzymes and if so what is the role of the cofactor/coenzyme in catalysis? c) How is...
Which statement(s) is(are) true about carbonic anhydrase enzyme? A. Carbonic anhydrase enzyme is involved in the...
Which statement(s) is(are) true about carbonic anhydrase enzyme? A. Carbonic anhydrase enzyme is involved in the production of chemicals that are important for controlling the blood pH of humans, B. Coordination site of the metal in carbonic anhydrase aquires a square planar geometry C. Histidine groups attached to the metal get polarized during the catalytic process. A and B A and C Band C B A QUESTION 7 Determine the d electron configuration of [Fe(NH 3) 61 3* complex (tag)Pleglo...
8. (4 pts) The biological catalyst carbonic anhydrase converts carbon dioxide to carbonic acid, an essential...
8. (4 pts) The biological catalyst carbonic anhydrase converts carbon dioxide to carbonic acid, an essential component of the bicarbonate buffer system regulating our blood pH. What mass (in me) of sodium carbonate (NaHCO, MM: 84.006 g/mol) must be added to 500.0 ml of 0.029 M carbonic acid (K. 1 4.3 x 10-7, K., 4.8 x 10-11) to produce a solution with pH = 7.40? Assume no volume change after the addition of sodium carbonate. H2CO3 + H2O=HCO3 + H20...
to which hard acid or hard base will carbonic anhydrase stick? .Carbonic anhydrases has zinc-containing enzymes.
to which hard acid or hard base will carbonic anhydrase stick? .Carbonic anhydrases has zinc-containing enzymes.
Carbonic anhydrase has two potential substrates: CO2 and HCO3 - . The KM value for CO2...
Carbonic anhydrase has two potential substrates: CO2 and HCO3 - . The KM value for CO2 is 12 mM and the KM for HCO3 - is 26 mM. Which substrate does carbonic anhydrase have a higher affinity for?
This image represents a close up view of the active site of carbonic anhydrase. The spherical...
This image represents a close up view of the active site of carbonic anhydrase. The spherical Zn^2+ ion is coordinated to three amino acids (part of the anhydrase structure), each one connected through a nitrogen atom. A fourth coordination site is now bonded to a hydroxide ion. This hydroxide used to be a water molecule. Thinking back to how ions can affect the pH of water, (Chapter 15, acid/base properties of salts), how did water become hydroxide in this instance?...
how does hot and cold temperatures affect the enzyme carbonic anhydrase
how does hot and cold temperatures affect the enzyme carbonic anhydrase
What's the relationship between CO2, bicarbonate (HC03- ), and carbonic acid (H2CO3)? In what form is...
What's the relationship between CO2, bicarbonate (HC03- ), and carbonic acid (H2CO3)? In what form is most CO2 carried in our blood? What is the role of carbonic anhydrase in gas exchange?
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of...
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of carbonic acid. The following are the initial rate data for this reaction which were obtained in a 1-mL solution that contained 0.0025 gram of carbonic anhydrase (mw -29,100 Da): CO2] (mM) Vo (mM/min) 0.88 1.55 4 2.47 16 3.54 32 4.50 5.21 64 (a) Plot these data in a Lineweaver-Burke plot. Label both axes including proper units. (b) Determine KM, Vmax and the...
15. Compare the catalytic activity of of the following enzymes: carbonic anhydrase, aspartate transcarbomylase (ATcase), restriction enzymes and myosins. (40 points) What is unique about each active site with regards to types of amino acid residues a) located in active site and interaction with substrate? b) Briefly what is the mechanism of catalysis in each active site? Are cofactors or coenzymes required by the enzymes and if so what is the role of the cofactor/coenzyme in catalysis? c) How is...
Which statement(s) is(are) true about carbonic anhydrase enzyme? A. Carbonic anhydrase enzyme is involved in the production of chemicals that are important for controlling the blood pH of humans, B. Coordination site of the metal in carbonic anhydrase aquires a square planar geometry C. Histidine groups attached to the metal get polarized during the catalytic process. A and B A and C Band C B A QUESTION 7 Determine the d electron configuration of [Fe(NH 3) 61 3* complex (tag)Pleglo...
8. (4 pts) The biological catalyst carbonic anhydrase converts carbon dioxide to carbonic acid, an essential component of the bicarbonate buffer system regulating our blood pH. What mass (in me) of sodium carbonate (NaHCO, MM: 84.006 g/mol) must be added to 500.0 ml of 0.029 M carbonic acid (K. 1 4.3 x 10-7, K., 4.8 x 10-11) to produce a solution with pH = 7.40? Assume no volume change after the addition of sodium carbonate. H2CO3 + H2O=HCO3 + H20...
This image represents a close up view of the active site of carbonic anhydrase. The spherical Zn^2+ ion is coordinated to three amino acids (part of the anhydrase structure), each one connected through a nitrogen atom. A fourth coordination site is now bonded to a hydroxide ion. This hydroxide used to be a water molecule. Thinking back to how ions can affect the pH of water, (Chapter 15, acid/base properties of salts), how did water become hydroxide in this instance?...
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of carbonic acid. The following are the initial rate data for this reaction which were obtained in a 1-mL solution that contained 0.0025 gram of carbonic anhydrase (mw -29,100 Da): CO2] (mM) Vo (mM/min) 0.88 1.55 4 2.47 16 3.54 32 4.50 5.21 64 (a) Plot these data in a Lineweaver-Burke plot. Label both axes including proper units. (b) Determine KM, Vmax and the...
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