to which hard acid or hard base will carbonic anhydrase stick? .Carbonic anhydrases has zinc-containing enzymes.
to which hard acid or hard base will carbonic anhydrase stick? .Carbonic anhydrases has zinc-containing enzymes.
15. Compare the catalytic activity of of the following enzymes: carbonic anhydrase, aspartate transcarbomylase (ATcase), restriction enzymes and myosins. (40 points) What is unique about each active site with regards to types of amino acid residues a) located in active site and interaction with substrate? b) Briefly what is the mechanism of catalysis in each active site? Are cofactors or coenzymes required by the enzymes and if so what is the role of the cofactor/coenzyme in catalysis? c) How is...
This image represents a close up view of the active site of carbonic anhydrase. The spherical Zn^2+ ion is coordinated to three amino acids (part of the anhydrase structure), each one connected through a nitrogen atom. A fourth coordination site is now bonded to a hydroxide ion. This hydroxide used to be a water molecule. Thinking back to how ions can affect the pH of water, (Chapter 15, acid/base properties of salts), how did water become hydroxide in this instance?...
Which statement(s) is(are) true about carbonic anhydrase enzyme? A. Carbonic anhydrase enzyme is involved in the production of chemicals that are important for controlling the blood pH of humans, B. Coordination site of the metal in carbonic anhydrase aquires a square planar geometry C. Histidine groups attached to the metal get polarized during the catalytic process. A and B A and C Band C B A QUESTION 7 Determine the d electron configuration of [Fe(NH 3) 61 3* complex (tag)Pleglo...
Carbonic anhydrase has two potential substrates: CO2 and HCO3 - . The KM value for CO2 is 12 mM and the KM for HCO3 - is 26 mM. Which substrate does carbonic anhydrase have a higher affinity for?
8. (4 pts) The biological catalyst carbonic anhydrase converts carbon dioxide to carbonic acid, an essential component of the bicarbonate buffer system regulating our blood pH. What mass (in me) of sodium carbonate (NaHCO, MM: 84.006 g/mol) must be added to 500.0 ml of 0.029 M carbonic acid (K. 1 4.3 x 10-7, K., 4.8 x 10-11) to produce a solution with pH = 7.40? Assume no volume change after the addition of sodium carbonate. H2CO3 + H2O=HCO3 + H20...
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of carbonic acid. The following are the initial rate data for this reaction which were obtained in a 1-mL solution that contained 0.0025 gram of carbonic anhydrase (mw -29,100 Da): CO2] (mM) Vo (mM/min) 0.88 1.55 4 2.47 16 3.54 32 4.50 5.21 64 (a) Plot these data in a Lineweaver-Burke plot. Label both axes including proper units. (b) Determine KM, Vmax and the...
Acetozolamide is a carbonic anhydrase inhibitor diuretic that is also used in the treatment of glaucoma. It can be administered intravenously four times a day, with each dose containing 250 mg of acetozolamide. If the solution has a concentration of 50 mg/ml, how many milliliters are required for a single dose?
11. Carbonic anhyd rase of red blood cells, which has a molecular weight of 30,000 is among the most active enzymes known. It catalyzes the reversible hydration of CO2 HCO,+ H H2O+ CO2 If 10 ug of pure carbonic anhyd rase catalyzes maximum hydration of 0.30 g of carbon dioxide in 1 min. at 37°C under optimal conditions, calculate the turnover number of carbonic anhydrase
Which reactions is catalyzed by carbonic anhydrase in the red blood cell when it reaches the lung? 1. H+ +HCO3->H2CO3 2. H2CO3 ---->H+ + HCO3- 3. H2CO3->CO2+H20 4. CO2 + H20 -> H2CO3
Describe the enzyme carbonic anhydrase by answering the following question: (a) Which reaction is catalyzed by this enzyme? (2 points) (b) Which cofactor is used by this enzyme? (1 point). (c) Which amino acids of the enzyme, and how many of them coordinate the cofactor? (2 points). (d) How does this cofactor help accelerate the reaction? Think about the chemistry (1 point) and the shift of the equilibrium (1 point).