Draw out the double strand break model of recombination, showing the parental and recombinant outcomes using...
Draw out the double strand break model of recombination, showing the parental and recombinant outcomes using the assignment provided in class. What phenotype would occur in cells with the following mutations: recA, RuvA, RuvB, Rec B
Homework Answers
Answer: (No assignment have been provided. So, the questions have been answered in general)
a. Please refer to the following figure:
b. recA mutant:
recA mutant cells have virtually no inheritance of markers in Hfr crosses. This represents a reduction of recombination of 100,000 fold. The RecA protein promotes synapsis and strand exchange between homologous DNA molecules. At least one of the DNA partners must be single-stranded.
RuvA and RuvB mutant:
RuvAB stimulate branch migration of DNA with the hydrolysis of ATP. Unlike the branch migration stimulated by the RecA protein, RuvAB-stimulated branch migration is equally efficient in either direction.
RuvA mutants that resolve HJs, have lost the capacity to reverse forks. In vivo and in vitro evidence indicate that the ruvA mutations affect DNA binding and the stimulation of RuvB helicase activity. RuvB mutants have been reported to affect branch migration.
RecB:
RecB reduce recombination in Hfr crosses (about 100-fold), but not as severely as recA. (The mutations are however epistatic with recA).
The recBCD protein is a complex enzyme catalyzing DNA unwinding (DNA helicase activity) and DNA degradation (exonuclease activity).
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Draw out the double strand break model of recombination, showing
the parental and recombinant outcomes using...
The results for the F2 progeny are shown for a F1 cross using the two X-linked...
The results for the F2 progeny are shown for a F1 cross using
the two X-linked markers:
w+ and m+.
As shown in the figure, the F1 cross is between the following
two parents:
- a phenotypically wild-type (red eye, full wing), heterozygous
female: w+m+/wm
- a double mutant male (white eye, miniature wing):
wm/Y
Answer the following questions:
1) In the F1 cross, can you infer the genotype for the phenotype
in the female? Why or why not?
2)...
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Please do not attempt if you are new to excel, an example has
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The results for the F2 progeny are shown for a F1 cross using
the two X-linked markers:
w+ and m+.
As shown in the figure, the F1 cross is between the following
two parents:
- a phenotypically wild-type (red eye, full wing), heterozygous
female: w+m+/wm
- a double mutant male (white eye, miniature wing):
wm/Y
Answer the following questions:
1) In the F1 cross, can you infer the genotype for the phenotype
in the female? Why or why not?
2)...
46-49 pleas help asap
ou employ CRISPR to target a DNA double strand break to your gene of interest. The break is then repaired via SA (Synthesis dependent strand annealing) using the sister chromatid as a template for repair. What change in DNA sequence would occur at your target gene? a. A deletion b. No change at all c. A change converting it to whatever allele is present on the homolog d. A chromosome arm exchange (crossover) with the homolog...
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