Question

The characteristics of the active site microenvironment of an enzyme can be largely independent of individual catalytic mechanisms. In general, active site microenvironments promote catalysis in which of the following ways? Choose one or more: o A. excluding excess water O B. providing salt bridges between the active site and the substrate in order to stabilize the interaction between the two O C. providing an optimized orientation of the substrate O D. decreasing the AG# of the reaction

Answer 1

Answer :- A, C , D

Explanation :- The active site of enzymes increase the rate of reaction because they decrease the activation energy of the reaction, and the physical microenvironment provides an optimal orientation of substrate relative to reactive functional groups while excluding excess water.

On the other hand some active sites may have amino acids that form salt Bridges with the amino acids from a substrate, not all do , so this is not a genetic strategy or ways of active site microenvironment.

So the answer is A,C,D.

Answer 2

c. Provide optimized orientation

active site obtimize orientation o (substate) tume + A Product Inzyme

An active site of an enzyme provide proper orientation of the substrate to come and sit on it, which is crucial for formation of product.

This has been established by Lock-kry hypothesis depicted in the answer. the active site helps in adopting the proper orientation of the substrate, which is very important for reaction to take place.