Question

Analysis of plant pigments lab report

Please help me with making a graph and answering the following questions

Thanks!

of eai wil be taken at 450 nm) and re-zero the machine nce valuit reading e ament at 400 nm, you can then 11) After finding the absorbance value wavelength by 50 nm (so the next reading wil h using the cuvette with just solvent as you did before en at 450 You Cn pigment and record your results. 12) Repeat this process, increasing by 50 nm and re-zeroi complete 700 nm. 13) Fill out the table below with your measured absorbance values should be put in as zero. Ethans20 A number in the lavI Then,m and rien mdease the astare d the the machine each time, untl you , Negative values near zero Absorbance Wavelength (nm) Carotene Chlorophyll a Chlorophyll b Xanthophyl Anthocyanin 400

*anthocyanin is excluded becasue we couldn’t obtain the data

Answer 1

0.25 0.2 0.15 1 0.1 0.05 0 400 550 650 500 600 700 450 0.05 Wavelength CaroteneChlorophyll aChlorophyll b xanthophyll Anthocyanin

1) In the graph we can observe that chlorophyll a and b shows an absorption spectrum between 400-450 and 650-700. This is coherent because the established absorption spectrum for both is between of the blue and orange-red light (around 450-475 nm and 650-675 nm). On the other hand, the rest of pigments showed just one peak between 420-440 which means that the only are excited by one interval of wavelengths.

2) I would conclude that It's beneficial for a plant to have more than one pigment because the more energy they can absorb from the sun using their pigments, the better levels of photosynthesis. Sometimes, plants are not in contact with a specific wavelength, for that reason there are mora than one pigments capable of absorb energy