List two key component parts of a plasmid required for use in recombinant DNA cloning
List two key component parts of a plasmid required for use in recombinant DNA cloning
Homework Answers
Two key component parts of a plasmid required for use in recombinant DNA cloning are:
1) Multiple cloning sites:Short segment of DNA which contains several restriction sites which helps in easy insertion of DNA.In expression plasmids,the MCS is often downstream from a promoter.
2) Selectable marker:The antibiotic resistance gene allows for selection in bacteria.However, many plasmids also have selectable markers for use in other cell types.
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List two key component parts of a plasmid required for use in
recombinant DNA cloning
Sub-cloning is a powerful technique that involves the preparation of a recombinant plasmid containing a DNA...
Sub-cloning is a powerful technique that involves the preparation of a recombinant plasmid containing a DNA fragment of interest and subsequent transformation that plasmid. In this experiment, you preformed a transformation, but the recombinant plasmid containing phage DNA was already prepared. Describe how you would make a recombinant plasmid containing a fragment you have already generated by PCR , including what enzymes you would use and the approximate length of time each step would take.
RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To...
RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To begin the recombinant DNA process, scientists must first ide at codes for the production of the protein they want to manufacture. One is to go backwards from the amino acid sequence of the desired protein to ide sequence of the gene. After scientists have identified the gene, they m it. Restriction enzymes or endonucleases from bacterial cells are key in th ia produce restriction...
A plasmid containing recombinant DNA is introduced into a bacteria. This plasmid is cloned during... a....
A plasmid containing recombinant DNA is introduced into a bacteria. This plasmid is cloned during... a. metaphase I b. binary fission c. the S phase of interphase d. mitosis
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid"...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid"...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
7. Explain the procedure for cloning DNA fragment into the plasmid PBR322 (shown on the right) (S...
7. Explain the procedure for cloning DNA fragment into the plasmid PBR322 (shown on the right) (S pts.). The gene fragment of interest was obtained by digestion of chromosomal DNA with the restriction enzyme Sall and subsequent purification using agarose gel electrophoresis. Which antiblotic would you use in the final step of the cloning procedure, and Pst why? EcoR Sal Ampicillin Tetracycline resistanica(Ter Amp) PBR322 4,361 bp) Origin of replicatiorn (ori Pvull 8. Assume that your gene fragment from question...
Describe 2 meiotic sources of recombination? Why is plasmid based engineered DNA considered recombinant?
Describe 2 meiotic sources of recombination? Why is plasmid based engineered DNA considered recombinant?
The first publications describing the successful production and intracellular replication of recombinant DNA appeared in the...
The first publications describing the successful production and intracellular replication of recombinant DNA appeared in the early 1970’s. Since then, there is a long list of breakthroughs achieved by recombinant DNA technology. But which one of the following are NOT on that list? Choose all that are NOT on the list (can be more than 1 option, or maybe its just 1) Group of answer choices Reproductive cloning of over a dozen humans Recombinant Factor VIII protein for the treatment...
RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To begin the recombinant DNA process, scientists must first ide at codes for the production of the protein they want to manufacture. One is to go backwards from the amino acid sequence of the desired protein to ide sequence of the gene. After scientists have identified the gene, they m it. Restriction enzymes or endonucleases from bacterial cells are key in th ia produce restriction...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
7. Explain the procedure for cloning DNA fragment into the plasmid PBR322 (shown on the right) (S pts.). The gene fragment of interest was obtained by digestion of chromosomal DNA with the restriction enzyme Sall and subsequent purification using agarose gel electrophoresis. Which antiblotic would you use in the final step of the cloning procedure, and Pst why? EcoR Sal Ampicillin Tetracycline resistanica(Ter Amp) PBR322 4,361 bp) Origin of replicatiorn (ori Pvull 8. Assume that your gene fragment from question...
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