During translation and transcription of a eukaryotic gene in a bacteria.
What would happen if GTPase (enzyme that converts GTP to GDP) activity was blocked? Why?
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During translation and transcription of a eukaryotic gene in a bacteria. What would happen if GTPase...
What are the differences between eukaryotic and bacterial transcription? Bacteria can do a unique co-transcriptional translation while eukaryotes normally are not able to. Why is so?
5. The tight coupling of translation with transcription in bacteria makes possible what mechanism of gene regulation (e.g., at the trp operon) that is not possible in eukaryotes? Just name the mechanism.
In eukaryotic cells, there are specific transcription factors (activators) that regulate gene expression. Describe the organization of eukaryotic genes (i.e. promoters/enhancers) and how transcription factors are able to control gene expression and enzyme activity. a) Throughout the course we discussed the enzyme lactase and how it is differently regulated (and therefore expressed) in different human populations. Describe how this gene is differently expressed in different populations and when this/these change(s) are predicted to have taken place. b) Differential gene expression...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
E: For each of the following, indicate whether they regulate eukaryotic gene expression at the transcription level (prior to or during transcription), at the translation level(after transcription/formation of the immature mRNA molecule), or both. 1. Addition of the signal-recognition particle (SRP) 2. DNA methylation 3. Enhancers or insulators
E: For each of the following, indicate whether they regulate eukaryotic gene expression at the transcription level (prior to or during transcription), at the translation level (after transcription/formation of the immature mRNA molecule), or both. 1. Alternative splicing 2. Chromatin remodeling 3. RNAi 12pt v Paragraph Β Ι Ο Α ev T²w ...
Eukaryotic genes can be introduced into bacteria by recombinant DNA techniques. If the introduced gene encodes a protein that is also found in bacteria—for example, a universally used glycolysis enzyme—then expression of the eukaryotic gene may produce a protein that functions in the bacterial cell. In an experiment, the entire mouse gene for a glycolysis enzyme, including its promoter, coding regions and termination sequence, is introduced into an E. coli cell that has a mutant gene for the bacterial version...
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
Uluruunu us RJ15 1. Draw or describe the process of eukaryotic transcription and translation, using the following terms as needed (not all terms will be used): sigma factor, RNA polymerase, DNA polymerase, origin of replication, ribosome, start codon, transcriptional start site, stop codon, nucleus, -10 and -35 sequences, TATA box, TBP, inducer, transcriptional stop site, Shine-Delgrano sequence, Kozak sequence, RNA splicing. 2. Draw or describe the process of prokaryotic/eubacterial transcription and translation, using as many of the terms above as...
What would happen to the expression of this gene if this cell did not have any GAL4 protein and why? What would happen to the expression of this gene if this cell did not have any GAL80 protein and why? What would happen to the expression of this gene if this cell did not have any GAL3 protein and why? Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down...