1. You are adding 40mL of apple juice to 360mL of water. What is the dilution...
1. You are adding 40mL of apple juice to 360mL of water. What is the dilution factor?
2. You are diluting your bacterial culture twice; 10-1 and 10-2. What is the overall dilution factor?
3. How would you prepare a 1/2 dilution? a 1/5 dilution?
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1. You are adding 40mL of apple juice to 360mL of water. What is
the dilution...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Question 3: You want to prepare a fruit punch by mixing your apple juice with some...
Question 3: You want to prepare a fruit punch by mixing your apple juice with some pineapple juice and water. 1. You want the punch to contain 40 mg of glucose from the apple juice. Which volume of apple juice should you measure? Show your calculations below. 2. You now want to add water to bring the volume to a final volume of 50 mL. How much water do you need to add? Show your calculations below. 3. You are...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...
QUESTION 4 You carry out a dilution adding 20 ml of solution A and 80 mL...
QUESTION 4 You carry out a dilution adding 20 ml of solution A and 80 mL of water. This dilution can NOT be expressed as: o 1 in 4 dilution. O 1:4 dilution. 5 fold dilution. having a dilution factor of 5. 1 in 5 dilution.
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
QUESTION 4 You carry out a dilution adding 20 ml of solution A and 80 mL of water. This dilution can NOT be expressed as: o 1 in 4 dilution. O 1:4 dilution. 5 fold dilution. having a dilution factor of 5. 1 in 5 dilution.
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
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