Question

hello I will be performing an experiment in microbiology lab testing 4 different Bathroom cleaners. I will be using S. aureus bacteria. what other materials should i be using? and what method should i follow? please don't just say use nutrient agar!
thank you in advance!

Answer 1

material: sterile media: nutrient agar plates              (3) divide the petri dish into four parts to test each bathroom cleaner in each part and one for control.

                                 sterile swabs                        (3)

                                 sterile bathroom cleaner samples         (5mL each)

                                 sterile circular absorbent paper disk      (5- 4 for cleaners and 1 for control)

                                culture of S.aureus.

miscellanoeus: bunsen flame

                     alcohol beaker

                    tweezer

Method used : disk diffusion method. first swab the plates with S.aureus culture under sterile conditions.

                    dip the disc into each of the cleaner solutions under antiseptic conditions.

                    using a tweezer place the disc containing the soaked bathroom cleaner onto the center of each quadrant with respective labelling of the cleaner.

                   on control plate swab the S.aureus culture and place a disc dipped in sterile water.

                    incubate the plates at 37oC for 24 hrs.

                   after 24 hrs measure the zone of inhibition around the bathroom cleaners and measure the zone of inhibition.