Question

Match the 6 enzymatic transformations listed above the bins, each with the one reaction it most closely Map.. resembles, in terms of mechanism and cofactor(s). Each bin will have exactly one correct answer, and each answer fits best in just one bin. Malate to Oxaloacetate Isocitrate dehydrogenase reaction Succinate to Fumarate Pyruvate to Oxaloacetate Glyoxalate condensation with acetyl-CoA G lycogen phosphorylase 2 AcetylcoA form acetoacetyl-CoA 6 -phosphogluconate to ribulose-5-phosphate Acetyl-CoA to Malonyl-CoA Catabolism of a deoxyribonucleoside to form deoxribose-1-phosphate and a free base Glucose-6P to 6-phosphogluconate Acyl CoA to trans-A2-enoyl CoA

Answer 1

Ans :

1) Malate to oxaloacetate - Glucose 6 phosphate to 6 phospho gluconate.

Malate is oxidised by malate dehydrogenase to oxaloacetate by producing NADH. Glucose 6 phosphate is oxidised to 6 phospho gluconate by glucose 6 phosphate dehydrogenase producing NADPH.

2)Isocitrate dehydrogenase reaction - 6-Phosphogluconate to ribulose 5 phosphate.

Isocitrate dehydrogenase and is an enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2. 6 phospho gluconate is oxidised to an intermediate which is decarboxylated to form ribulose 5 phosphate.

3) Pyruvate to oxaloacetate - Acetyl coA to malonyl CoA. Pyruvate is converted to oxaloacetate by pyruvate carboxylase. Acetyl coA is converted to malonyl coA by acetyl coA carboxylase.

4) Glycogen phosphorylase - Catabolism of deoxyribonucleoside to form deoxy ribose 1 phosphate and a free base.

Glycogen phosphorylase produces glycogen (mer) + glyucose 1 phosphate in the reaction.

5)Glycosylation condensation with acetyl coA - Acetyl CoA from acetoacetyl CoA.

Both involves acetyl coA transferases.

6)Succinate to fumarate -Acetyl coA to trans delta enoyl coA.