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For the following list of peptides, predict which peptide would elute from the column first. Note that all conditions below a

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Not changed since last attempt Marked out of 2.00 Question 5 Which peptide would elute last from a column that separates ONLY

Not changed since last attempt Marked out of 2.00 Question 6 Which peptide would be the last to elute from an anion exchange

Not changed since last attempt Marked out of 2.00 Question7 Predict which peptide would be the last to leave the column in a

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Answer #1

5.) A

Sequence A has more hydrophobic amino acids as compared to other sequence, so on the basis of hydrophobicity , this sequence would be the last to elute out.

6.) B

Anion exchange chromatography , means matrix is anion exchanger ( has + charge) , and it binds to anion.

Sequence B would be the last to elute because it has more amino acids which has negatively charged side chains.

7.) E, this is correctly marked.

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