Use answers from answer bank and place in the correct bins.
Solution :
we know that the pH > pl then protein has net negative charge and its get binded to anion exchange coloumn and when the pH < pl then peptide has net positive charge and peptide gets binded to cation exchange column
Anion exchange for protein A
a) pH = 3.0, Peptide A (pI = 5.0), Positive charge --> no bind
b) pH = 6.9, Peptide A (pI = 5.0), Negative Charge --> binds
c) pH = 10.5, Peptide A (pI = 5.0), Negative charge --> binds
Canion exchange for protein A
a) pH = 3.0, Peptide A (pI = 5.0), Positive charge --> binds
b) pH = 6.9, Peptide A (pI = 5.0), Negative Charge --> will not bind
c) pH = 10.5, Peptide A (pI = 5.0), Negative charge --> will not bind
For Protein B; Anion Exchanger:
a) pH = 3.0, Peptide B (pI = 9.2), Positive charge --> no bind
b) pH = 6.9, Peptide B (pI = 9.2), Positive charge --> no bind
c) pH = 10.5, Peptide B (pI = 9.2), Negative charge --> will bind
For Protein B; Canion Exchanger:
a) pH = 3.0, Peptide B (pI = 9.2), Positive charge --> binds
b) pH = 6.9, Peptide B (pI = 9.2), Positive charge --> binds
c) pH = 10.5, Peptide B (pI = 9.2), Negative charge --> no bind
Use answers from answer bank and place in the correct bins. Peptides can be separated using...
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