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uestion 3 of 9 > Peptides can be separated using an ion-exchange column based on their...
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which...
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pl of 9.2, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0 cation-exchange column at pH = 3.0 Answer Bank peptide B pI = 9.2 peptide A...
Use answers from answer bank and place in the correct bins. Peptides can be separated using...
Use answers from answer bank and place in the correct bins.
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pl of 9.2, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0 cation-exchange column at pH...
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH-...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
16) At which pH values would two different peptides, one with a pl of 5.9 and...
16) At which pH values would two different peptides, one with a pl of 5.9 and the other with a pl of 9.1, both bind to a cation exchange column? A) pH 3.9 B) pH 7.3 C) pH 10.9 D) None of the above 17) Which type of gel matrix (aka resin) would separate the following proteins using size exclusion chromatography: protein 1 (2,000 Da), protein 2 (7,000 Da), protein 3 (20,000 Da), and protein 4 (90,000 Da)? A) Gel...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
#2 is Q HiTrap column where it is anion exchange column not cation 2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrin...
#2 is Q HiTrap column where it is anion exchange column not
cation
2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
M4. Water Softening Using Ion Exchange Method It is desired to remove Ca ions from water...
M4. Water Softening Using Ion Exchange Method It is desired to remove Ca ions from water using ion exchange technique. For this purpose, water, initially containing 700 mg Ca2+/L, is passed through a column filled with sulphonated styrene-based cation exchange resin. The breakthrough curve of this ion exchange process is given in Figure 1. 141 a) Show the breakthrough point and also the point, where the exchange capacity of the resin is exhausted, on the same graph. [3] b) How...
5. When all of the sample to be collected has left the column, will the drop...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
NOTES: To separate on a size-exclusion column, differences in MW need to be greater than 10%....
NOTES: To separate on a size-exclusion column, differences in MW need to be greater than 10%. To separate proteins by charge, the difference in pI must be at least 0.5 pH unit. Consider the table of four proteins below: Protein Size pI Size exclusion Charge at pH 7.4 DEAE-elution 1 49 kDa 6.8 2 51 kDa 8.1 3 53 kDa 6.4 4 99 kDa 8.3 5 106 kDa 3.8 6 143 kDa 7.9 (a) In what order would the proteins...
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pl of 9.2, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0 cation-exchange column at pH = 3.0 Answer Bank peptide B pI = 9.2 peptide A...
Use answers from answer bank and place in the correct bins.
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pl of 9.2, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0 cation-exchange column at pH...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
16) At which pH values would two different peptides, one with a pl of 5.9 and the other with a pl of 9.1, both bind to a cation exchange column? A) pH 3.9 B) pH 7.3 C) pH 10.9 D) None of the above 17) Which type of gel matrix (aka resin) would separate the following proteins using size exclusion chromatography: protein 1 (2,000 Da), protein 2 (7,000 Da), protein 3 (20,000 Da), and protein 4 (90,000 Da)? A) Gel...
#2 is Q HiTrap column where it is anion exchange column not
cation
2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
M4. Water Softening Using Ion Exchange Method It is desired to remove Ca ions from water using ion exchange technique. For this purpose, water, initially containing 700 mg Ca2+/L, is passed through a column filled with sulphonated styrene-based cation exchange resin. The breakthrough curve of this ion exchange process is given in Figure 1. 141 a) Show the breakthrough point and also the point, where the exchange capacity of the resin is exhausted, on the same graph. [3] b) How...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
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