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Could I get some help on how i could answer this question. MW- 42685.15 pI =...

Could I get some help on how i could answer this question.

MW- 42685.15

pI = 6.68 # amino acids = 393

Use the calculated molecular weight of the protein to determine the amount of your protein required to make 5 mL of a10 μM solution for further fictitious spectroscopic analysis.

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Answer #1

Calcilate no.of mole of protein required to make 10 micromole 5 ml solution

And then calculate gm of protein from no.of mole amd MW.

All formula and calculation given in following image

ume of sal in tihes Up:af mal ro tein rzquired to make … 0 e tein solution 2equixth Те, max. 40 ro bein

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