You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate...
You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate proteins into mitochondria. So far you have eliminated any of the proteins forming the various transporters (TOM complex, SAM complex, TIM22 complex, TIM23 complex and OXA complex) and the porin proteins as candidates for the mutation. In other words, all the transporter proteins are wild type.
What is your hypothesis for a protein that may be responsible for this mutant phenotype.
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You have isolated a temperature-sensitive mutant that, at the
restrictive temperature, has no ability to translocate...
You are a scientist studying DNA replication. You have the ability to introduce temperature-sensitive mutations into...
You are a scientist studying DNA replication. You have the ability to introduce temperature-sensitive mutations into the bacteria that you are studying (temperature-sensitive mutations work normally at one temperature, but when you raise the temperature a little, the mutation exerts its effect. This allows you to grow colonies with mutations that would otherwise be lethal. The fact that these mutations are temperature sensitive has nothing to do with the problem, other than to make it accurate to how these would...
As a student project, you have isolated six new mutant strains of E. coli with altered...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
1. You are characterizing a temperature-sensitive mutant called nlk1 in Saccharomyces cerevisiae. To examine whether the...
1. You are characterizing a temperature-sensitive mutant called nlk1 in Saccharomyces cerevisiae. To examine whether the mutant has a defect in cell cycle progression, you perform two experiments. First, you try flow cytometry with samples of the mutant strain incubated at the permissive or non-permissive temperatures A wildtype strain incubated at each temperature is a used as control. The flow cytometry results are shown below in Panel A. Second, you examine the Nlk1 protein levels throughout the cell cycle in...
Please help :( 11. (20 points) You have isolated two mutants of a normally pear-shaped microorganism...
Please help :(
11. (20 points) You have isolated two mutants of a normally pear-shaped microorganism that have lost their distinctive shape and are now round. One of the mutants has a defect in a protein that you call A and the other has a defect in a protein that you call B. First, you grind up each type of mutant cell and normal cells separately and separate the plasma membranes from the cytoplasm, forming the first cell extract. Then...
Inspired by Lee Hartwell's Nobel Prize (2001), you decide to study cell cycle regulation in yeast....
Inspired by Lee Hartwell's Nobel Prize (2001), you decide to study cell cycle regulation in yeast. You know that the expression of a cell cycle-associated kinase, Cdk24p, changes throughout the cell cycle, being very high in G 1/S and low in G2/M. Nevertheless, mRNA levels for CDK24 are constant throughout the cell cycle. You decide to study how this regulation occurs. You isolate a temperature sensitive mutant cell linethat fails to divide at the non-permissive temperature and notice that Cdk24p...
n 13 You have found a mutant that has a G-protein that can only bind GDP...
n 13 You have found a mutant that has a G-protein that can only bind GDP and cannot bind GTP. Which of the following might you expect in the mutant cells? red ed out of ag question Select one: a. The secondary messenger would be made constantly. b. The cells would have more energy availability due to higher GTP binding. c. The secondary messenger would not be made. O d. The primary messenger would not be able to bind to...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
1. You are characterizing a temperature-sensitive mutant called nlk1 in Saccharomyces cerevisiae. To examine whether the mutant has a defect in cell cycle progression, you perform two experiments. First, you try flow cytometry with samples of the mutant strain incubated at the permissive or non-permissive temperatures A wildtype strain incubated at each temperature is a used as control. The flow cytometry results are shown below in Panel A. Second, you examine the Nlk1 protein levels throughout the cell cycle in...
Please help :(
11. (20 points) You have isolated two mutants of a normally pear-shaped microorganism that have lost their distinctive shape and are now round. One of the mutants has a defect in a protein that you call A and the other has a defect in a protein that you call B. First, you grind up each type of mutant cell and normal cells separately and separate the plasma membranes from the cytoplasm, forming the first cell extract. Then...
n 13 You have found a mutant that has a G-protein that can only bind GDP and cannot bind GTP. Which of the following might you expect in the mutant cells? red ed out of ag question Select one: a. The secondary messenger would be made constantly. b. The cells would have more energy availability due to higher GTP binding. c. The secondary messenger would not be made. O d. The primary messenger would not be able to bind to...
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