Question

You purify bee (Apis mellifera). After a year of research, you determine that the enzyme is essential for a pathway that allows the organism to produce venom. You are now interested in how the enzyme is regulated, so you conduct a series of experiments to help you address this aim. You finally determine that the enzyme is inhibited under conditions that prevent its dephosphorylation. What do these results suggest about the mechanism of regulation of beesting? a new enzyme (beesting) from the Western honey A. The enzyme is positively regulated by proteolytic cleavage B. The enzyme is positively regulated by a protein kinase C. The enzyme is negatively regulated by a protein kinase D. The enzyme is negatively regulated by phosphatase protein а

The answer is C, can someone please explain why this is the answer?

Answer 1

Kinases are enzymes that phosphorylate a substrate. Phosphatases are enzymes that dephosphorylate a substrate.

In the question it is written that, enzyme is inhibited by the conditions that prevents its dephosphorylation, it means when dephosphorylation of enzyme does not occur, the enzyme does not work. So, we can say that enzyme is negatively regulated by kinanses.

From the above statement it can be said that enzyme is activated when it is dephosphorylated. It means enzyme is positively regulated by phosphatases.

Negatively regulated means that the enzyme is inhibited and positively regulated means that the enzyme is activated.

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