Homework Answers
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
20) Six tubes are in a rack.. You mix 1ml of cell culture with 0.5 ml...
20) Six tubes are in a rack.. You mix 1ml of cell culture with 0.5 ml of water in tube #1, transfer 0.1 ml of tube #1 to tube #2 containing 0.9ml of water and mix well and the same procedure (0.1 ml of culture +0.9 ml of water) is repeated for the remaining 4 tubes. After plating 0.1 ml of the last dilution tube #6, you obtain 100 colonies. what is the cell density (cfu/ml) of the original cell...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
these are my absorbance numbers after dilution. How do i figure out my plots using beers...
these are my absorbance numbers after dilution.
How do i figure out my plots using beers law? NOT USING EXCEL Im
required to use the graph which is in the last picture.
*please read the lab or dont answer* thank you!
Amax- Procedure: Part B: Beer's Law 1. Wash, rinse and dry four medium-sized test tubes. 2. Into the first test tube, pour 10 mL of the stock solution. 3. Into tube #2, pipet 5 mL of H20 and 5...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL...
The concentration of a solution after dilution can be found by: where M1 is the concentrated...
The concentration of a solution after dilution can be found
by:
where M1 is the concentrated solution (in
this lab, 0.400 M), and V1 is the volume of the
concentrated solution placed in the test tube. The
V2 is the total volume of
concentrated solution plus water added, and M2
is the concentration of the solution after diluting.
Tube 1 has 2.00 mL of the 0.400 M Ni2+ solution and
8.00 mL of water added. What is the concentration of...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml...
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml (stock) and then prepared 1:100 dilutions as a working solution (tube A). The company also suggest verifying the optimal dilution for your experiment by doing serial (twofold) dilutions. Describe how you would prepare 500microliters of working solution (tube A). What volume of inhibitor will you add in microliter? 2) You would like to optimize the working concentration of the inhibitor, and therefore perform two-fold...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume of culture plate In plate 10-4 there were 150 colonies counted. Experimental process: Pipet 0.25 mL of the S. cerevisiae solution into the test tube labeled 10-1. Pipet the solution up and down several times to ensure all organisms are rinsed from the pipet into the test tube. Pipet 0.25 mL of the 10-1 S. cerevisiae solution into the test tube labeled 10-2. Pipet...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
these are my absorbance numbers after dilution.
How do i figure out my plots using beers law? NOT USING EXCEL Im
required to use the graph which is in the last picture.
*please read the lab or dont answer* thank you!
Amax- Procedure: Part B: Beer's Law 1. Wash, rinse and dry four medium-sized test tubes. 2. Into the first test tube, pour 10 mL of the stock solution. 3. Into tube #2, pipet 5 mL of H20 and 5...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL...
The concentration of a solution after dilution can be found
by:
where M1 is the concentrated solution (in
this lab, 0.400 M), and V1 is the volume of the
concentrated solution placed in the test tube. The
V2 is the total volume of
concentrated solution plus water added, and M2
is the concentration of the solution after diluting.
Tube 1 has 2.00 mL of the 0.400 M Ni2+ solution and
8.00 mL of water added. What is the concentration of...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
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