Define the terms cis and trans in terms of mechanisms regulating eukaryotic gene expression.
Define cis-acting elements and trans-acting factors. How do they in a eukaryotic cell?
Choose the cis-elements. Click on the cis regulatory elements only. Control of eukaryotic gene expression (gene control) can occur at multiple levels from DNA accessibility to post-translational changes to polypeptides/proteins. Silencers, repressors, transcription factors, activators, enhancers, insulators histone acetyltransferases (HATS), histone deacetylases (HDACs) histone remodellers and DNA methyltransferases all play a role in gene control.
6. Experiments comparing the expression and silencing mechanisms of cloned eukaryotic gene "A" via the lac operon versus the trp operon would best be accomplished using: (1 point) OPCR O nucleic acid hybridization O electroporation O a YAC and expression vectors, each clipped with a different restriction enzyme O a BAC and expression vectors, all clipped with the same restriction enzyme O a YAC and expression vectors, all clipped with the same restriction enzyme
Define gene expression. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Gene expression is producing a from a gene sequence (usually of a gene).
Choose two (2) of the mechanisms of gene expression regulation in eukaryotic cells denoted by rows shown (7 possible in the Figure below. I will only grade your first to for completeness and will NOT grade any more that you write. If you do an EXTRAODINARY job on your answers, you may ear bonus points For each of your choices answer the following 4 questions using COMPLETE sentences 1. What are the base structural differences between molecules (pink, blue or...
Which of the following is not true regarding regulation of gene expression in eukaryotes? In eukaryotic cells, transcriptional activators often work by recruiting the transcriptional macinery to gene promoters DNA methylation is an example of an epigenetic mark that can be inherited Acetylation of histones can result in a more open chromatin structure to allow transcription to occur Different genes can be turned off or on between different organs or stages of development Transcription factor DNA sequences are bound by...
The CFTR gene is a eukaryotic gene, but how is the gene structured in terms of the numbers and lengths of exons and introns? "Sketch" a rough, but labelled diagram of the exon/intron structure of the CFTR gene and explain the significance of exons and introns and what such gene structures mean in terms of gene function. As part of the explanation, address the relationship between allelic variants and gene structure
Define constitutive gene expression and differential gene expression.
3. Mutations in bacteria that affect gene regulation may have a cis- or trans-effect on the expression of the protein-encoding genes in an operon? How would the following mutations affect the expression of the lac operon? Assume there could be more than one lac operon in the cell and that no glucose is present. A. A mutation in the operator site that prevents the lac repressor from binding. B. A mutation in the lacI gene that prevents allolactose from binding,...
In the GAL gene system, __________ are cis-acting regulatory elements, and __________ protein is a trans-acting regulatory protein.