Define constitutive gene expression and differential gene expression.
Define constitutive gene expression and differential gene expression.
Two-step model of constitutive unregulated gene expression. a) Write down the corresponding system of coupled ordinary differential equations (ODEs) based on mass action kinetics. Identify and explain which biological/biochemical process the “source” and “sink” terms in each equation describe. b) State the parameters for steady-state mRNA and protein levels. OM promoter (A) mRNA (M) protein (P) Figure 1. Two-step model of constitutive (unregulated) gene expression. Here the promoter (A) is a constant and represents a gene.
Define gene expression. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Gene expression is producing a from a gene sequence (usually of a gene).
Define the terms cis and trans in terms of mechanisms regulating eukaryotic gene expression.
Define Mendel's law of independent assortment. A) The expression of one gene has no effect on the expression of another gene. B) Segregation of homologous chromosomes is random. C) The inheritance of one character has no effect on the inheritance of another character. D) There are two versions of each trait, a dominant and a recessive; the one you get is random.
1. Define the steps of gene expression. Be sure to specifically speak to the following: (USL05.1) DNA Replication Transcription Translation
1. Define the steps of gene expression. Be sure to specifically speak to the following: (USLO5.1) DNA Replication Transcription Translation 2. Discuss vertical versus horizontal transmission.
Which of the following mutations could lead to constitutive expression of the genes of the lac operon?
Generally, describe how the sequencing data was analyzed for differential gene expression. How was it determined that genes were differentially expressed using DESeq? 2. 3. At the end of the DESeq pipeline, you got a list of 1000 differentially expressed genes. Which command would have changed the number of differentially expressed genes? How would it have done so?
How does differential gene expression control organismal development? All cells are differentiated early in development, and that determines the final fate of the cell. Epigenetic inheritance controls what genetic material ends up in each individual cell, determining its fate. Each cell contains different genes that are controlled by gene regulators and determine the final fate of the cell. Development is controlled by genes regulated in different ways in different cells and at different times during development.
The constitutive promoter that you placed upstream of your gene is expressing too much of the gene. Which of the following can you use to have it express less? ☐ Make the promoter weaker by changing the sequences of the -10 and -35 regions to their reverse complements ☐ Make the promoter stronger by changing the sequences of the -10 and -35 regions to more closely match the consensus sequences ☐ Make the promoter weaker by changing the sequences of...