Which of the following mutations could lead to constitutive expression of the genes of the lac...
Which of the following mutations could lead to constitutive expression of the genes of the lac operon?
Homework Answers
Mutation in the operator sequence could lead to constitutive expression of the genes of the lac operon because this prevents the binding of repressor. Without binding of repressor, the gene stays activated under all conditions.
Add Answer to:
Which of the following mutations could lead to constitutive
expression of the genes of the lac...
Imagine you are carrying out research on the lac operon. You isolate six mutations in the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following...
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following statements is consistent with the expression phenotype? genome: I+P+ O- Z+ Y- plasmid: I+ P- O+ Z- Y+ constitutive expression of β-gal and permease induced in the presence of lactose constitutive expression of β-gal only constitutive expression of β-gal and permease β-gal and permease induced in the presence of lactose constitutive expression of permease and β-gal induced in the presence of lactose
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
I am very confused on these questions. Please be as accurate as possible to recieve all...
I am very confused on these questions. Please be as accurate as possible to recieve all points. 1. Which of the following mutations could lead to constitutive expression of the genes of the lac operon? A) A mutation in the operator sequence B) A mutation in the lac-Z gene C) A mutation in the lac-Y gene D) A super repressor mutation 2. Which of the following best describes the biological role of the lac operon? A) It ensures that a...
For the lac genotypes shown, predict whether the structural genes (Z) are constitutive, permanently repressed, or...
For the lac genotypes shown, predict whether the
structural genes (Z) are constitutive, permanently
repressed, or inducible in the presence of lactose
For the lac genotypes shown, predict whether the structural genes (Z) are constitutive, permanently repressed, or inducible in the presence of lactose
In the lac operon, what are the likely effects on operon transcription of the mutations listed...
In the lac operon, what are the likely effects on operon transcription of the mutations listed below? PLEASE EXPLAIN your answers. I think I answered the first part of the question but need help explaining why. Mutation of the consensus sequence in the lac promoter. i. Transcription is blocked. Mutation of the repressor binding sites in the operator sequences. i. Transcription is constitutive. Mutation of the lacI gene affecting the allosteric site of the protein. i. Transcription is blocked. Mutation...
3. Mutations in bacteria that affect gene regulation may have a cis- or trans-effect on the...
3. Mutations in bacteria that affect gene regulation may have a cis- or trans-effect on the expression of the protein-encoding genes in an operon? How would the following mutations affect the expression of the lac operon? Assume there could be more than one lac operon in the cell and that no glucose is present. A. A mutation in the operator site that prevents the lac repressor from binding. B. A mutation in the lacI gene that prevents allolactose from binding,...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon of E. coli leads to an inability to ferment lactose, and the expression of the operon is always off. Introduction into the mutant of an F' factor containing the wild type lac operon does NOT restore the ability to ferment lactose, i.e., there is no expression of the operon from the plasmid either. a. What is the probable nature of Mutation A? Is the...
4. Briefly draw and/or describe the functions of the genes contained in the lac operon. Why...
4. Briefly draw and/or describe the functions of the genes contained in the lac operon. Why is a low level of lac operon expression necessary for it to respond to the presence of lactose ?
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
For the lac genotypes shown, predict whether the
structural genes (Z) are constitutive, permanently
repressed, or inducible in the presence of lactose
For the lac genotypes shown, predict whether the structural genes (Z) are constitutive, permanently repressed, or inducible in the presence of lactose
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
Most questions answered within 3 hours.
-
Based on how much or how little you have noticed in the media,
do you think...
asked 2 hours ago -
I calculate Rosetta Stone’s P/E ratio as 10x prior to a report
about future industry growth....
asked 3 hours ago -
What major physiological changes account for electrodermal
activity (as in during a lie-detector test)?
asked 4 hours ago -
You are going to sell fruits & vegetables at a local
roadside market. List separately and...
asked 4 hours ago -
Hows does water affect the digestion of fats ?
asked 7 hours ago -
A buffer that contains 0.41 M of a base, B and 0.21 M of its
conjugate...
asked 7 hours ago -
Pleaaaaaaaasssssse help me please please it's more important
:((((((((((((((((((((
CASE STUDY
Nissan Company
Nissan Motor Company,...
asked 7 hours ago -
Calculate the concentration of hydronium and hyroxide ions in 1)
2.0 mol/L HNO3 2) 0.5 mol/L...
asked 7 hours ago -
In a loop-the-loop ride a car goes around a vertical, circular
loop at a constant speed....
asked 7 hours ago -
50 grams each of water (18.015 g/mol) and ethylene glycol
(C2H6O2) (62.07 g/mol) are mixed. At...
asked 7 hours ago -
The life expectancy of sport utility vehicles follows a normal
distribution with a mean of 145.8...
asked 8 hours ago -
If an object with mass of 5 kg is pushed with a force of 552 N,...
asked 7 hours ago