For the lac genotypes shown, predict whether the structural genes (Z) are constitutive, permanently repressed, or inducible in the presence of lactose
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For the lac genotypes shown, predict whether the
structural genes (Z) are constitutive, permanently
repressed, or...
A) Given the following genotypes of E. coli strains, predict whether the lacZ gene will be...
A) Given the following genotypes of E. coli strains, predict whether the lacZ gene will be inducibly expressed, constitutively expressed, or permanently repressed in the presence of lactose as the only sugar. Genotype I+O+Z+ will have the lacZ gene __________ , because __________ I-O+Z+ will have the lacZ gene __________ , because __________ ISO+Z+ will have the lacZ gene __________ , because __________ I+OCZ+ will have the lacZ gene __________ , because __________ ISOCZ+ will have the lacZ gene __________ , because __________ B) The following strains of E. coli carry a...
A) Given the following genotypes of E. coli strains, predict whether the lacZ gene will be...
A) Given the following genotypes of E. coli strains, predict whether the lacZ gene will be inducibly expressed, constitutively expressed, or permanently repressed in the presence of lactose as the only sugar. Genotype I+O+Z+ will have the lacZ gene __________ , because __________ I-O+Z+ will have the lacZ gene __________ , because __________ ISO+Z+ will have the lacZ gene __________ , because __________ I+OCZ+ will have the lacZ gene __________ , because __________ ISOCZ+ will have the lacZ gene __________ , because __________
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following...
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following statements is consistent with the expression phenotype? genome: I+P+ O- Z+ Y- plasmid: I+ P- O+ Z- Y+ constitutive expression of β-gal and permease induced in the presence of lactose constitutive expression of β-gal only constitutive expression of β-gal and permease β-gal and permease induced in the presence of lactose constitutive expression of permease and β-gal induced in the presence of lactose
UT EID: D. 5 E. 6 13. For the E. coli strain containing the following alleles...
UT EID: D. 5 E. 6 13. For the E. coli strain containing the following alleles of the lac operon, expression of lacZ and lacY is inducible, constitutively on or permanently repressed. (erepressor; r cannot bind operator: osoperator, o cannot bind repressor, lac are LOF mutations) A. lacZ is inducible, lacY is constitutively on. B. lacZ is constitutively on, lacY is inducible. C. lacZ is permanently repressed, lacY is constitutively on. D. lacZ is constitutively on, lacY is permanently repressed....
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
In the table below, predict (yes or no) whether or not the E. coli lac operon...
In the table below, predict (yes or no) whether or not the E. coli lac operon will be transcriptionally active in the presence or absence of glucose or lactose as indicated and respond to questions "a" and "b." (20 points) Lactose Glucose Lac expression? No Yes NO Yes Yes NO Yes No YES 1) Explain each of your answers in terms of the molecular mechanisms that are known to underlie the regulation of the lac operon. 2) Which mechanism is...
UT EID: D. 5 E. 6 13. For the E. coli strain containing the following alleles of the lac operon, expression of lacZ and lacY is inducible, constitutively on or permanently repressed. (erepressor; r cannot bind operator: osoperator, o cannot bind repressor, lac are LOF mutations) A. lacZ is inducible, lacY is constitutively on. B. lacZ is constitutively on, lacY is inducible. C. lacZ is permanently repressed, lacY is constitutively on. D. lacZ is constitutively on, lacY is permanently repressed....
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
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