Question

The amino acid sequence of a portion of a polypeptide is
N...Gly-Glu-Met-Tyr-Trp-Lys-Ala...C.

I just need help with part C

A. Determine the mRNA sequence encoding this polypeptide fragment. 5'-G-G-N-G-A-Pu-A-U-G-U-A-Py-U-G-G-A-A-Pu-G-C-N-3'

B. Determine the DNA template strand sequence corresponding to the mRNA above. 5' N-G-C-Py-T-T-C-C-A-Pu-T-A-C-A-T-Py-T-C-N-C-C- 3'

C. Determine the DNA coding strand sequence corresponding to the template strand above.

Express your answer as a sequence of nucleotides separated by dashes. Use N to represent any nucleotide (A, T, C or G), Pu to represent a purine( A, G), and Py to represent a pyrimidine (C, T). Example: C-T-Pu-N-.

Answer 1

DNA template strand = 3' to 5'

DNA coding strand = 5' to 3'

Both the strands are antiparallel and complementary to each other. Purine (A, G) will pair with pyrimidine (T, C).

So, coding strand sequence will be,

3' N C G Pu A A G G T Py A T G T A Pu A G N G G 5'

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