Cell Biology Short Answer Question: In the absence of targeting information, what is the default location...
Cell Biology Short Answer Question: In the absence of targeting information, what is the default location of proteins synthesized on cytosolic ribosomes? What are the general features of an N-terminal signal sequence that targets secretory proteins to the ER? What are the general features of mitochondrial matrix-targeting sequences?
Homework Answers
Cytosol is the defalt location of proteins synthesized on cytosolic ribsomes in the abasence of targeting information
the general features of an N-terminal signal sequence that targets secretory proteins to the ER are the N-teminal signials sequence that targets secretory proteins to ER would be roughly 16 -30amino acids with with roughly 6-12 amino acids that are hydrophobic
the general features of mitochondrial matrix-targeting sequences-
1.located N-terminus
2.20-50 aminoacids in lenth
3.Rich in hydrophobic aminoacids, positively charged amino acid( Arg, lys), and hydroxylated ones (ser,thr)
4.lack negitvely charged acidic residue (Asp, Glu)
5.Alpha-helical conformation(one-hydrophobic,opposite side -charge amino acid: amphipathic)
6.Amphipathicity of matrix -targeting sequences is critical their function.
Add Answer to:
Cell Biology Short Answer Question: In the
absence of targeting information, what is the default location...
The import of proteins into mitochondria requires energy in the form of ATP for their [____]...
The import of proteins into mitochondria requires energy in the form of ATP for their [____] and of mitochondria that are energized, with an electrochemical gradient. The imported proteins must be synthesized on [____] ribosomes as they are bound to requisite [____]. Protein import itself requires that the protein have an N-terminal targeting sequence that folds into an [____] for interaction with the import receptor on the outer mitochondrial membrane. The translocase of the outer membrane (TOM) complex consists of...
1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal)...
1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal) 2. Name 4 organelles that require organelle specific targeting sequences in polypeptide 3. Name 3 final locations for proteins with ER signal sequence 4. Satranslational translocation: (a) Name the protein that the signal sequence binds to (b) Name the protein SRP binds to 5. State the function of signal peptidase 6. Name a membrane protein that is not a single pass membrane protein 7....
Problem 4.1.3 (tags: #protein targeting, #protein topology, #transfer sequence) a) Make a map showing the location...
Problem 4.1.3 (tags: #protein targeting, #protein topology, #transfer sequence) a) Make a map showing the location of signal sequences, start transfer sequences and stop transfer sequences in protein A and protein B below that end up inserted in the membrane as shown. Protein A NH, Cytosol NA J B Scoot & Membrane Protein B COOH HAN Ν - FC ER Cisterna b) Make a map for the arrangement of signal sequences, start transfer sequences and stop transfer sequences for a...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein (GFP). This means that the GFP will be targeted and glow at the organelle of interest and they can use these to mark different organelles during fluorescence microscopy. To make these marker proteins, they take the sequence of GFP and add the desired sorting signal. A. GFP on its own is an excellent protein to use as an organelle marker protein because it is...
For her research project, Anneka found this great website to predict the transmembrane domains of secretory...
For her research project, Anneka found this great website to predict the transmembrane domains of secretory proteins. An algorithm allows estimating the hydrophobicity of amino acid stretches in the sequence of secretory proteins. Hydrophobic stretches are plotted with positive values on the Y-axis. On the X-axis, Anneka can see the protein sequence expressed as amino acid number, with N-terminus at the intersection with the Y-axis. Example of prediction of TMD domains (a) Human growth hormone receptor (type 1) Nw Signal...
Please answer all questions 2 After isolating the rough endoplasmic reticulum from the rest of the...
Please answer all questions
2 After isolating the rough endoplasmic reticulum from the rest of the cytoplasm, you purify the RNAS attached to it. Which of the following proteins do you expect the RNA from the rough endoplasmic reticulum to encode? (a) (c) soluble secreted proteins plasma membrane proteins ER membrane proteins all of the above (b) (d) -13 In which cellular location would you expect to find ribosomes translating MRNAS that encode ribosomal proteins? (a) (c) the nucleus in...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the...
genetic biology 5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need...
genetic biology
5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need to extend the short stretch of nucleotides shown on the bottom strand? (b) Write the sequence of the newly synthesized fragment and label its S' and 3' end. (c) The covalent bond between these adjacent nucleotides is what type of chemical bond? After using a chemical mutagen to generate mutations in a DNA sequence, scientists noted a mutation from C to T at the...
SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question....
SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question. 00 OA Figure 2.1 Using Figure 2.1, match the following: 1) Tertiary (protein) structure, 2) Monosaccharide 3) Nucleotide 4) Lipid 5) Functional protein 6) Polymer 7) Polysaccharide TRUE FALSE. Write T if the statement is true and F if the statement is false, 8) Final preparation for cell division is made during the cell life cycle subphase called G2. SHORT ANSWER. Write the word...
1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal) 2. Name 4 organelles that require organelle specific targeting sequences in polypeptide 3. Name 3 final locations for proteins with ER signal sequence 4. Satranslational translocation: (a) Name the protein that the signal sequence binds to (b) Name the protein SRP binds to 5. State the function of signal peptidase 6. Name a membrane protein that is not a single pass membrane protein 7....
Problem 4.1.3 (tags: #protein targeting, #protein topology, #transfer sequence) a) Make a map showing the location of signal sequences, start transfer sequences and stop transfer sequences in protein A and protein B below that end up inserted in the membrane as shown. Protein A NH, Cytosol NA J B Scoot & Membrane Protein B COOH HAN Ν - FC ER Cisterna b) Make a map for the arrangement of signal sequences, start transfer sequences and stop transfer sequences for a...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein (GFP). This means that the GFP will be targeted and glow at the organelle of interest and they can use these to mark different organelles during fluorescence microscopy. To make these marker proteins, they take the sequence of GFP and add the desired sorting signal. A. GFP on its own is an excellent protein to use as an organelle marker protein because it is...
For her research project, Anneka found this great website to predict the transmembrane domains of secretory proteins. An algorithm allows estimating the hydrophobicity of amino acid stretches in the sequence of secretory proteins. Hydrophobic stretches are plotted with positive values on the Y-axis. On the X-axis, Anneka can see the protein sequence expressed as amino acid number, with N-terminus at the intersection with the Y-axis. Example of prediction of TMD domains (a) Human growth hormone receptor (type 1) Nw Signal...
Please answer all questions
2 After isolating the rough endoplasmic reticulum from the rest of the cytoplasm, you purify the RNAS attached to it. Which of the following proteins do you expect the RNA from the rough endoplasmic reticulum to encode? (a) (c) soluble secreted proteins plasma membrane proteins ER membrane proteins all of the above (b) (d) -13 In which cellular location would you expect to find ribosomes translating MRNAS that encode ribosomal proteins? (a) (c) the nucleus in...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the...
genetic biology
5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need to extend the short stretch of nucleotides shown on the bottom strand? (b) Write the sequence of the newly synthesized fragment and label its S' and 3' end. (c) The covalent bond between these adjacent nucleotides is what type of chemical bond? After using a chemical mutagen to generate mutations in a DNA sequence, scientists noted a mutation from C to T at the...
SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question. 00 OA Figure 2.1 Using Figure 2.1, match the following: 1) Tertiary (protein) structure, 2) Monosaccharide 3) Nucleotide 4) Lipid 5) Functional protein 6) Polymer 7) Polysaccharide TRUE FALSE. Write T if the statement is true and F if the statement is false, 8) Final preparation for cell division is made during the cell life cycle subphase called G2. SHORT ANSWER. Write the word...
Most questions answered within 3 hours.
-
Which of the following pairs of chemicals could be used to make
a buffer solution?
A.)...
asked 10 minutes ago -
You purchase your daily coffee at the Chemist’s Coffeehouse and
have a choice of two cups:...
asked 23 minutes ago -
Problem 16-51 Sales
Activity Variance (LO 16-3)Odessa, Inc., reports the following
information concerning operations for the...
asked 48 minutes ago -
Pictured on the right are thee point charges Q1 = 18.4 μC, Q2 =
-30.6 μC,...
asked 50 minutes ago -
A graduate student is conducting research in psychology and
needs to obtain the IQ scores of...
asked 1 hour ago -
R2.84: There are 2 defective products in a production lot of 10.
An inspector randomly selected...
asked 2 hours ago -
Consider the following equilibrium system: COCl2(g) CO(g) +
Cl2(g) A 10.00 L evacuated flask is filled...
asked 3 hours ago -
1) What are the two distinct steps that one needs to perform
when developing a data...
asked 3 hours ago -
2) Write a C++ program that uses a class called “Degree” to
obtain the trigonometric
values...
asked 4 hours ago -
1. In eukaryotic cells the genomes of
&
asked 4 hours ago -
The standard enthalpy of propanol (C3H7OH) is -303.0 kJ/mol.
Compute both of the
gross and net...
asked 4 hours ago -
Why PWM using H-bridge for control motor speed is more power
effiecient than the linear amplifier...
asked 4 hours ago