Question

QUESTION 15 What is the purpose of adding sodium dodecyl sulfate (SDS) to the protein mixture when running polyacrylamide gel electrophoresis? it induces transcription Oit denatures the proteins into a linear structure it labels the protein with a black color so it can be viewed after the it migrates through the gel O it catabolizes the protein into individual amino acids

Answer 1

15. The correct answer is it denatures the proteins into a linear structure. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis, or SDS-PAGE for short, is the technique where proteins are denatured and linearized, then run across a current through a thin gel, which separates the proteins by size. SDS is a detergent with a strong protein-denaturing effect and binds to the protein backbone at a constant molar ratio. In the presence of SDS and a reducing agent that cleaves disulfide bonds critical for proper folding, proteins unfold into linear chains with negative charge proportional to the polypeptide chain length. Thus, SDS makes the protein in linearized form so that it can run in SDS-PAGE.