Question

20. Part of the mix. Your frustrated colleague hands you a mixture of four proteins with the following properties: Protein A Protein B Protein C Protein D isoelectric point (pl) 4.1 9.0 8.8 3.9 Molecular weight (in kDa) 80 81 37 172 (a) Propose a method for the isolation of Protein B from the other proteins. (b) If Protein B also carried a His tag at its N-terminus, how could you revise your method?

Answer 1

a) Method for the isolation of protein b from the outer proteins = > 2 d get electroscopic it is a key techniques for separation of protein complex according to their isoelectric points an molecular weight. b) if protein b carrier a this tag at its n term the method would be - as chromatography with column made up of NI - NTA as histidine binos with high efficiency with ni^2 + ions in the ni - nta columns. As a result in protein b will be within the column an we can illute protein b with high cone of salts passing through the column.