Question

Question 1 10 pts Explain how you would use DNA sequence data from two related organisms (like a mouse and human) to find a functional gene (10 points): HTML Editor Paragr

Answer 1

We can use CpG islands to detect the functional gene in related organisms using DNA sequences.

One characteristic shared by ∼50% of mammalian promoters is the occurrence of CpG islands located near the 5’ ends of genes. We can therefore searched the entire human and mouse gene clusters for CpG islands using the CpG plot program.

Detection of regions of genomic sequences that are rich in the CpG pattern is important because such regions are resistant to methylation and tend to be associated with genes which are frequently switched on. Regions rich in the CpG pattern are known as CpG islands. The function of the program CpG Plot is to plot CpG rich areas, and CpG Report to report all CpG rich regions.