8. You want to design a plasmid vector that would enable researchers to have their favorite...
8. You want to design a plasmid vector that would enable researchers to have their favorite protein(s) secreted by E. coli, even though that protein is not normally a secreted protein. Which of the secretion systems would you choose and why? Describe what this plasmid vector would entail.
Homework Answers
Add Answer to:
8. You want to design a plasmid vector that would enable
researchers to have their favorite...
2. You are trying to design a protein that will be expressed in Escherichia coli and...
2. You are trying to design a protein that will be expressed
in Escherichia coli and secreted outside of the cell for
purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
cell. Which secretion system would work best for this project?
Support your answer with evidence based on the properties of E.
coli, the protein, and the secretion system. (20 pts)
2. You are trying to design a...
You are trying to design a protein that will be expressed in Escherichia coli and secreted...
You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system
You are trying to design a protein that will be expressed in Escherichia coli and secreted...
You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E,coli is a gram-negative cell and the protein folds after it has existed the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E.coli, the protein , and the secretion system.
A fellow researcher is constructing a plasmid vector they are hoping to express in e. coli....
A fellow researcher is constructing a plasmid vector they are hoping to express in e. coli. As they are designing their experimental protocols they are concerned that they will not be able to identify colonies which were successfully transformed, with the correct plasmid/insertion construct. Provide a suggested mechanism or method to address their concerns. Specifically answer the following two questions and provide details on the biological mechanism underpinning the method. a. Describe a mechanism to determine if the bacterial have...
NOTE: this will require that you remember some stuff from bacterial genetics. You want to research...
NOTE: this will require that you remember some stuff from
bacterial genetics.
You want to research the eukaryotic protein BabyFeet, which
smells like peppermint. To make cDNA you need...
a) You prepare plates to grow your bacteria on. Your competent
E. coli are resistant to penicillin, cephalexin, and susceptible to
ampicillin. Which antibiotic will you put in your media?
b) Next, you prepare your cloning vector, which contains the
genes Ampr, cepr, and penr all downstream of the promoter. The...
You have cloned the Pepsi Gene into the vector pKEN. You used two restriction enzymes, BamHI...
You have cloned the Pepsi Gene into the vector pKEN. You used two restriction enzymes, BamHI and EcoRI to force the cloning in a directional way. The plasmid (pKEN) is ~5 kb long and the insert Pepsi 800bp long. After transformation into E. coli you grow up several colonies and isolate the vector. After digestion of the vector it looks like you have three potential clones. You need to sequence the gene to make sure you have the correct clone....
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...
Suppose you have a dna fragement you would like to insert into the psap plasmid. the...
Suppose you have a dna fragement you would like to insert into the psap plasmid. the fragement has pstl and ecorl restriction endonuclease sites near the 5' end and hindlll and smal restriction endonuclease sites near the 3' end. choose the best restriction endonuclease(s) to digest the both the dna fragment and psap.
You want to make E. coli bacteria that glow green. Your plan is to insert the...
You want to make E. coli bacteria that glow green. Your plan is to insert the gene for green fluorescent protein (GFP) from a jellyfish (a eukaryote) into the genome of the bacteria so that the mRNA is transcribed by the E. coli cells and translated into protein. What changes do you need to make to the jellyfish GFP gene sequence to make sure that the bacteria will make a functional protein? Why? (1 point) A type of amatoxin binds...
BINF4000 chapter 7, question 6 You have a protein sequence and you want to know iis...
BINF4000 chapter 7, question 6 You have a protein sequence and you want to know iis structure in a short time. you perform BLAST and PSI-BLAST searches of the PDB and you find a sequence with 15% amino acid identity to your protein. The e-value is .5. Which of the following options would you choose to achieve your goal. A. Use X-ray crystallography B. Use NMR spectroscopy C. Submit your sequence to a protein structure prediction server that performs homology...
2. You are trying to design a protein that will be expressed
in Escherichia coli and secreted outside of the cell for
purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
cell. Which secretion system would work best for this project?
Support your answer with evidence based on the properties of E.
coli, the protein, and the secretion system. (20 pts)
2. You are trying to design a...
NOTE: this will require that you remember some stuff from
bacterial genetics.
You want to research the eukaryotic protein BabyFeet, which
smells like peppermint. To make cDNA you need...
a) You prepare plates to grow your bacteria on. Your competent
E. coli are resistant to penicillin, cephalexin, and susceptible to
ampicillin. Which antibiotic will you put in your media?
b) Next, you prepare your cloning vector, which contains the
genes Ampr, cepr, and penr all downstream of the promoter. The...
Suppose you have a dna fragement you would like to insert into the psap plasmid. the fragement has pstl and ecorl restriction endonuclease sites near the 5' end and hindlll and smal restriction endonuclease sites near the 3' end. choose the best restriction endonuclease(s) to digest the both the dna fragment and psap.
Most questions answered within 3 hours.
-
Does smoking hurt the lungs of children’s who are exposed to
adult smoking?
Forced vital capacity...
asked 28 minutes ago -
EXPLAIN HOW NEGATIVE AFFECTIVITY IS RELATED TO JOB SATISFACTION.
INCLUDE AN EXAMPLE. MUST BE ANSWERED TWO...
asked 43 minutes ago -
On December 31, 2019, Lincoln Inc. sold a used industrial crane
for $660,000 cash. The original...
asked 1 hour ago -
a runner jogs 3.4 miles every morning. how many kilometers does
this represent
asked 2 hours ago -
A 60.80 gram sample of iron (with a heat capacity of 0.450
J/g◦C) is heated to...
asked 2 hours ago -
Question 1 2 pts
The preprocessor executes after the compiler.
False
True
Question 2 2 pts...
asked 3 hours ago -
Explain how a spinal cord injury above C3 could result in a
respiratory arrest
asked 3 hours ago -
You are a statistician and wish to estimate, with 90%
confidence, the proportion of adults who...
asked 6 hours ago -
A man is standing 3.40 m in front of a convex spherical mirror
of radius of...
asked 6 hours ago -
Match the annual percentage rate to each of these trade credit
terms:
__ 1/5, NET 60...
asked 6 hours ago -
ORGANIC CHEMISTRY QUESTION 5
PART A--------
Describe a chemical test for the identification of a double...
asked 9 hours ago -
Both Terence and Tong work at a local actuarial consulting firm
in Des Moines.
Terence arrives...
asked 10 hours ago