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You decide to use PCR to determine if your genotype for the PTC tasting gene TAS2R38....

You decide to use PCR to determine if your genotype for the PTC tasting gene TAS2R38. You then decide to also determine genotype for another gene called PER3. What PCR "ingredient" would be different in these two tests?

Select one:

a. Template gDNA

b. dNTPS (nucleotides)

c. Taq DNA polymerase

d. Primers

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Answer #1

d. Primers.

primers are short segment of DNA which required for DNA polymerase to synthesize DNA. In PCR two primers are used and these are forward and reverse primer and they are design in such a way that they flank the specific region of genome so that specific region of DNA is synthesize. So to determine genotype for TAS2R38 gene, primers are used which flank the TAS2R38 gene and for PER3 gene primers which flank the PER3 gene are used, here for two different gene different types of primers are used. But template genomic DNA used is of single type, dNTPs are universal (A,G,T,C) and taq DNA polymerase is also same it only require 3,OH end free to add nucleotide. So here answer is primers.

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