MEIOSIS IN HUMAN :-
In humans, meiosis is the process by which sperm cells and egg cells are produced. In the male, meiosis takes place after puberty. Diploid cells within the testes undergo meiosis to produce haploid sperm cells with 23 chromosomes. A single diploid cell yields four haploid sperm cells through meiosis.
In females, meiosis begins during the fetal stage when a series of diploid cells enter meiosis I. At the conclusion of meiosis I, the process comes to a halt, and the cells gather in the ovaries. At puberty, meiosis resumes. One cell at the end of meiosis I enters meiosis II each month. The result of meiosis II is a single egg cell per cycle (the other meiotic cells disintegrate). Each egg cell contains 23 chromosomes and is haploid.
The union of the egg cell and the sperm cell leads to the formation of a fertilized egg cell with 46 chromosomes, or 23 pairs. Fertilization restores the diploid number of chromosomes. The fertilized egg cell, a diploid, is a zygote. Further divisions of the zygote by mitosis eventually yield a complete human being.
POLYMERASE CHAIN FREACTION :-
Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make millions to billions of copies of a specific DNA sample allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1983 by Kary Mullis.
DNA polymerase occurs naturally in living organisms. In cells it functions to duplicate DNA when cells divide in mitosis and meiosis. Polymerase works by binding to a single DNA strand and creating the complementary strand.
Describe human meiosis in detail. What is polymerase chain reaction and how was it used in...
Describe how the polymerase chain reaction (PCR) works. What are the three steps in a PCR cycle and what is happening at each step?
9. What is polymerase chain reaction and how is it applied in recombinant DNA technologies?
Describe in detail the structure of the RNA polymerase II CDT and how the structure is used to sequentially regulate the maturation of the pre-mRNA during the elongation phase of transcription. Focus on the events which happen on RNA polymerase II CDT. For mechanisms which are directly responsible for pre-mRNA processing, you only need to mention their names.
polymerase chain reaction to amplify a 500 bp region of human dna corresponding to a gene of interest. when your pcr reaction is complete, you plan to run the product on an agarose gel to determine if your pcr reaction worked correctly. 1. Explain how you going to make the pcr soup and why you going to make it this way. 2. State the what's in the control and why the controls are so important
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR) is a powerful tool for amplifying genetic material (that is, making many copies of it). This technique can be used for a wide range of applications, from examining and manipulating the specific genes involved in making L. monocytogenes pathogenic to analyzing the phylogenetic relationships between microbes. What are the steps of PCR required to amplify the recombinant DNA? Drag and drop the events into...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Can someone help me figure out this concept map of Polymerase Chain Reaction. Microbiology Polymerase Chain Reaction Match the terms on the right to the correct box below Polymerase chain reaction which are amplifies Specific sequences of DNA involves the following three steps facilitated by use of Cooling to -65°C Denaturation Extension Primers Priming Raising temperature to -72 Raising temperature to -910 Repeated in multiple cycles Taq polymerase Target DNA Target DNA Thermocycler accomplished by accomplished by accomplished by separates...
The polymerase chain reaction was originally designed/run using the polymerase from E. coli. What type of organisms gave rise to the 'new' class of polymerases that streamlined the PCR procedure? Psychrophiles Thermophiles Alkalophiles Acidophiles
Question 32 (2 points) The Polymerase Chain Reaction is used to: O a) amplify a small amount of DNA Ob) separate DNA fragments by size Oc) identify target plasmids O d) seal "sticky ends" e) cleave bacterial plasmids
1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C. a.Why would such a heat-stable polymerase be beneficial in PCR? b.What would happen if it weren’t heat stable? c.How might you choose...