Answer:
a). Amplify a small amount of DNA
Explanation:
Polymerase chain reaction (PCR) amplify the small amount of DNA.
Gele electrophoresis = Separate DNA fragments by size
Seal “sticky ends” by ligase enzyme.
Cleavage of bacterial plasmids by restriction enzyme.
Question 32 (2 points) The Polymerase Chain Reaction is used to: O a) amplify a small...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts of a target DNA sequence to be amplified. b) two primers, complementary to each end of the target sequence. c) a large supply of DNA nucleotides. d) a heat-stable DNA polymerase. e) All of the above
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The reaction is specific for certain sequences in the DNA. 2) Only small amounts of template are needed. 3) Results can only be obtained with freshly isolated DNA. 4) All the products from a specific part of the DNA will be the same size. Question 2: Saving The chain terminator used in Sanger's DNA sequencing is works due to.. 1) lack of 2'OH 2) ddNTP:...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Polymerase chain reaction (PCR) can be used for many different purposes, including determining paternity. PCR amplifies specific DNA sequences from complex mixtures and can be used to amplify sequences that although they may not have any known function may have several unique sizes and these different forms are inherited according to the Law of Segregation. Below is a diagram of an agarose gel of PCR samples from a mother, and several children. Which letters represent children that could be blologically...
1. If you were trying to come up with an easy-to-remember analogy for the polymerase chain reaction, you would likely say PCR is similar to a colored pencils scissors dishwasher photocopier 2. What determines the piece of DNA that is amplified in PCR? The specific primers used The source of the DNA The temperature of each cycle The specific restriction enzyme used 3. What is the role of temperature in PCR? It is used to separate and anneal the nucleic...
RNA differs from DNA in that it: all of the above contains ribose sugar contains uracil is usually found as a single-stranded molecule in cells Question 21 pts The correct order of steps in a PCR cycle is: Extension, denaturation, annealing Annealing, extension, denaturation Annealing, denaturation, extension Denaturation, annealing, extension The goal of the polymerase chain reaction (PCR) is to: Amplify a small amount of DNA sequence Cleave DNA molecules Digest bacterial plasmids Sequence DNA Which of the following is...
Question Completion Status QUESTION 1 Choose the correct answer from the list. Answers will be used only once. The sequences at the ends of linear chromosomes are called A polymerase A protein that uses ATP hydrolysis to separate the two DNA strands during replication is called helicase Okazaki D telomeres The small segments of DNA synthesized on the lagging strand during replication are called fragments. E. Single-stranded binding protein Addition of deoxyribonucleotides to DNA is catalyzed by DNA must be...
QUESTION 3 The form of replication used by DNA is conservative. semiconservative. O dispersion. cumulo-nimbus QUESTION 4 The short fragments of the lagging strand are called Okazaki fragments. polymerase leading segments radioactivity molecules. QUESTION 5 0.5 points Which of the following are symbols for the four bases that are part of the make up of a DNA molecule? O A BT, G O CGAH O ATG,C O A U,G,C QUESTION 6 0.5 point In DNA replication, helicase is a protein...
28) le M ore Ullowing experiments transformation C) UV light mutation experiment B) Yeast Complementation experiment D) Transduction experiment 26) It red-colored flowers (Rare dominant over white colored flowers and wrinkled seeds (Ware dominant over smooth seeds, then the genotype RRww would appear as A) red, smooth ) white, smooth mooth red, wrinkled red, wrinkled D) white,wrinkled 29) Which of the following techniques is commonly used by Csi agents to solve crime, paternity cases, and identify victims, by matching human...