Describe how the polymerase chain reaction (PCR) works. What are the three steps in a PCR...
Describe how the polymerase chain reaction (PCR) works. What are the three steps in a PCR cycle and what is happening at each step?
Homework Answers
Answer:
The polymerase chain reaction is a process of DNA amplification which produces large copies of DNA molecules from the template strand.
There are 3 important steps of PCR
-
Denaturation: double-stranded DNA is separated into single-stranded DNA at high temperature (94C), this separation is necessary for primer binding
-
Annealing: Annealing of primers (short DNA fragments) complementary to 3’ end of the DNA at 54C.
-
Extension: Active DNA polymerase initiates polymerization synthesizing DNA over the template strand (72C).
Add Answer to:
Describe how the polymerase chain reaction (PCR) works. What are
the three steps in a PCR...
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR)...
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR) is a powerful tool for amplifying genetic material (that is, making many copies of it). This technique can be used for a wide range of applications, from examining and manipulating the specific genes involved in making L. monocytogenes pathogenic to analyzing the phylogenetic relationships between microbes. What are the steps of PCR required to amplify the recombinant DNA? Drag and drop the events into...
Explain the polymerase chain reaction and include all starting materials required, what happens in each cycle,...
Explain the polymerase chain reaction and include all starting materials required, what happens in each cycle, and how a scientific investigation might benefit from PCR.
Describe three steps of a cycle of PCR.
Describe three steps of a cycle of PCR.
Describe human meiosis in detail. What is polymerase chain reaction and how was it used in...
Describe human meiosis in detail. What is polymerase chain reaction and how was it used in this article?
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Primers are short pieces of DNA that are used in PCR (Polymerase Chain Reaction), the process...
Primers are short pieces of DNA that are used in PCR (Polymerase Chain Reaction), the process which is used in molecular labs to make copies of short stretches of a gene or genome. You have designed primes that have a Molecular Weight (MW) of 6000. It arrives as a dry sample in the quantity of 300 µg. How much water do you need to add to make a primer stock solution of 100 mM primers? HINTS: Remember that mM is...
1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts...
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts of a target DNA sequence to be amplified. b) two primers, complementary to each end of the target sequence. c) a large supply of DNA nucleotides. d) a heat-stable DNA polymerase. e) All of the above
polymerase chain reaction, many copies of regions of the genome for a number of different downstream...
polymerase chain reaction, many copies of regions of the genome for a number of different downstream applications. In this Project you are PCR-amplifying your mitochondrial HVR1 region so that you can obtain the DNA sequence for that region. The ultimate goal is to determine your haplotype or haplogroup based on that region, which will provide you with insight into your deep ancestry. consider the class discussions you had on PcR this week. Use this information to answer the following questions:...
help please Put the following steps to the Polymerase Chain Reaction in correc order 1st Tube...
help please
Put the following steps to the Polymerase Chain Reaction in correc order 1st Tube is heated to about 95 der Tube is cooled to 70 degrees Tag Polymerase reads the pare [Choose Tag Polym Tag Polymerase reads the parent DNA and attaches the correct nucleotides 5th Choose Hydrogen bonds between the two strands durature Whicco Tube is heated to about 95 degree Celcius 6th Choose The process starts with a segment of the DNA of Interest being added...
Polymerase chain reaction (PCR): The gold standard of nucleic acid amplification The polymerase chain reaction (PCR) is a powerful tool for amplifying genetic material (that is, making many copies of it). This technique can be used for a wide range of applications, from examining and manipulating the specific genes involved in making L. monocytogenes pathogenic to analyzing the phylogenetic relationships between microbes. What are the steps of PCR required to amplify the recombinant DNA? Drag and drop the events into...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts of a target DNA sequence to be amplified. b) two primers, complementary to each end of the target sequence. c) a large supply of DNA nucleotides. d) a heat-stable DNA polymerase. e) All of the above
polymerase chain reaction, many copies of regions of the genome for a number of different downstream applications. In this Project you are PCR-amplifying your mitochondrial HVR1 region so that you can obtain the DNA sequence for that region. The ultimate goal is to determine your haplotype or haplogroup based on that region, which will provide you with insight into your deep ancestry. consider the class discussions you had on PcR this week. Use this information to answer the following questions:...
help please
Put the following steps to the Polymerase Chain Reaction in correc order 1st Tube is heated to about 95 der Tube is cooled to 70 degrees Tag Polymerase reads the pare [Choose Tag Polym Tag Polymerase reads the parent DNA and attaches the correct nucleotides 5th Choose Hydrogen bonds between the two strands durature Whicco Tube is heated to about 95 degree Celcius 6th Choose The process starts with a segment of the DNA of Interest being added...
Most questions answered within 3 hours.
-
Which pickup should be purchased by Northwest Construction for
use by its job superintendent? How much...
asked 57 seconds ago -
Explain two features of the Affordable Care Act that are likely
to cause an increase in...
asked 3 minutes ago -
Organizational commitment is a person's attitudes towards
________.
his or her entire company
his or her...
asked 4 minutes ago -
3. Demand in a market dominated by two firms (a Cournot duopoly)
is determined according to:...
asked 4 minutes ago -
The Center for Disease Control and Prevention (CDC) estimated
in 2011 that 48 million people (1...
asked 6 minutes ago -
La
aceleracion centeipeda de un ciclista que esta viajando en una
trauectoria circular de radio 25...
asked 6 minutes ago -
During the period, Sanchez Company sold some excess equipment at
a loss. The following information was...
asked 10 minutes ago -
In a Union Find algorithm, why does path compression make
deunion, (undo union), hard?
How many...
asked 18 minutes ago -
1) Consider the hypotheses shown below. Given that x bar =113, σ
= 25, n=49, α=0.01,...
asked 24 minutes ago -
Suppose a company receives a windfall of cash. What factors should
the board of directors consider...
asked 25 minutes ago -
a. John has started to climb a steep flight of stairs, and
keeps climbing, and (phew!)...
asked 32 minutes ago -
Genetic
QUESTIONS FOR RESEARCH AND DISCUSSION
1. Discuss how incomplete penetrance, variable expressivity,
pleiotropy, and genetic...
asked 39 minutes ago