Experiment 3: Following Chromosomal DNA Movement through Meiosis
In this experiment, you will follow the movement of the chromosomes through meiosis I and II to create gam- etes.
Materials
Procedure Trial 1
As prophase I begins, the replicated chromosomes coil and condense...
1. Build a pair of replicated, homologous chromosomes. 10 beads should be used to create each individual sister chromatid (20 beads per chromosome pair). The five-holed bead represents the centromere. To do this...
For example, suppose you start with 20 red beads to create your first sister chromatid pair. Five beads must be snapped together for each of the four different strands. Two strands create the first chromatid, and two strands create the second chromatid.
Place the five-holed bead flat on a work surface with the node positioned up. Then, snap each of the four strands into the bead to create an “X” shaped pair of sister chromosomes.
Repeat this process using 20 new beads (of a different color) to create the second sister chromatid pair. See Figure 4 (located in Experiment 2) for reference.
Assemble a second pair of replicated sister chromatids; this time using 12 beads, instead of 20, per pair (six beads per each complete sister chromatid strand). Snap each of the four pieces into a new five-holed bead to complete the set up. See Figure 5 (located in Experiment 2) for reference.
Pair up the homologous chromosome pairs created in Step 1 and 2. DO NOT SIMULATE CROSSING OVER IN THIS TRIAL. You will simulate crossing over in Trial 2.
Configure the chromosomes as they would appear in each of the stages of meiotic division (prophase I and II, metaphase I and II, anaphase I and II, telophase I and II, and cytokinesis).
Diagram the corresponding images for each stage in the sections titled “Trial 1 - Meiotic Division Beads Diagram”. Be sure to indicate the number of chromosomes present in each cell for each phase.
Disassemble the beads used in Trial 1. You will need to recycle these beads for a second meiosis trial in Steps 7 - 11.
2 Sets of Different Colored Pop-it® Beads (32 of each - these may be any color)
4 5-Holed Pop-it® Beads (used as centromeres)
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Mitosis and Meiosis
Trial 1 Meiotic Division Beads Diagram
Prophase I
Metaphase I
Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase II
Cytokinesis
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Mitosis and Meiosis
Trial 2
8. Build a pair of replicated, homologous chromosomes. 10 beads should be used to create each individual sister chromatid (20 beads per chromosome pair). The five-holed bead represents the centromere. To do this...
For example, suppose you start with 20 red beads to create your first sister chromatid pair. Five beads must be snapped together for each of the four different strands. Two strands create the first chromatid, and two strands create the second chromatid.
Place the five-holed bead flat on a work surface with the node positioned up. Then, snap each of the four strands into the bead to create an “X” shaped pair of sister chromosomes.
Repeat this process using 20 new beads (of a different color) to create the second sister chroma- tid pair. See Figure 4 (located in Experiment 2) for reference.
Assemble a second pair of replicated sister chromatids; this time using 12 beads, instead of 20, per pair (six beads per each complete sister chromatid strand). Snap each of the four pieces into a new five-holed bead to complete the set up. See Figure 5 (located in Experiment 2) for reference.
Pair up the homologous chromosomes created in Step 8 and 9.
SIMULATE CROSSING OVER. To do this, bring the two homologous pairs of sister chromatids together (creating the chiasma) and exchange an equal number of beads between the two. This will result in chro- matids of the same original length, there will now be new combinations of chromatid colors.
Configure the chromosomes as they would appear in each of the stages of meiotic division (prophase I and II, metaphase I and II, anaphase I and II, telophase I and II, and cytokinesis).
Diagram the corresponding images for each stage in the section titled “Trial 2 - Meiotic Division Beads Diagram”. Be sure to indicate the number of chromosomes present in each cell for each phase. Also, in- dicate how the crossing over affected the genetic content in the gametes from Trial 1 versus Trial 2.
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Trial 2 Meiotic Division Beads Diagram:
Prophase I
Metaphase I
Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase II
Cytokinesis
Mitosis and Meiosis
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Answer:
II).
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Experiment 3: Following Chromosomal DNA Movement through Meiosis In this experiment, you will follow the movement...
Experiment 3: Following Chromosomal DNA Movement through Meiosis In this experiment, you will follow the movement of the chromosomes through meiosis I and II to create gametes Materials 2 Sets of Different Colored Pop-it® Beads (32 of each - these may be any color) 4 5-Holed Pop-it® Beads (used as centromeres) Procedure Trial 1 As prophase I begins, the replicated chromosomes coil and condense... Build a pair of replicated, homologous chromosomes. 10 beads should be used to create each individual...
Part 1 Build a pair of replicated, homologous chromosomes. Use ten beads to create each individual sister chromatid (20 beads per chromosome pair). Two five-holed beads represent each centromere. To do this: a. Start with 20 beads of the same color to create your first sister chromatid pair. Five beads must be snapped together for each of the four different strands. Two strands create the first chromatid, and two strands create the second chromatid, with a 5-holed bead at the...
Meiosis is composed of two chromosomal division called Meiosis I and Meiosis II. Each of the two divisions consists of Prophase, Prometaphase, Metaphase, Anaphase and Telophase and each of these phases is designated I or II according to which division it is a part of. Be sure you know what happens in each phase in regard to the chromosomes, chromatids, spindles and the nuclear envelope. During which phase does synapsis occur and crossing over begin? And in which division, Meiosis I or Meiosis II...
Simulate meiosis by drawing One pair of chromosomes and no crossing over Instructions: Set up your cell. The cell you start with should be: A diploid cell It should contain 1 pair of homologous chromosomes It should be in G1 of the cell cycle, before DNA replication occurs Be sure to show 2 genes that are both heterozygous . Go through the steps of meiosis. Be sure to show and label these in your pictures: Label homologous chromosomes and sister...
Can anyone help me with this? Thank you. Lab 13. Mitosis and Meiosis Purpose The purpose of this lab is to understand the process of mitosis and melosis in animal and plant cells. Learning Objectives After completing this lab, you will be able to: 1. Describe the stages of mitosis. 2. Identify the stages of mitosis in an onion root or whitefish blastula using a light microscope. 3. Describe how cytokinesis differs in plant vs. animal cells. 4. Name and...
Identify the stages of meiosis by filling in the blanks, and then rearrange the sentences to place the stages in the correct order starting with prophase I. Drag the text blocks below into their correct order. anaphase II Sister chromatids separate and are pulled to opposite poles of the cell during www telophase II metaphase wwwwwww Nuclear envelopes reform around two separate haploid nuclei during prophase www Chromosomes line up in pairs along the plate during anaphase Nuclear envelopes reform...
Question 3 Cell cycle progression is controlled by the rhythmic accumulation and degradation of:Question 3 options:CyclinsCyclin-dependent kinases (Cdks)BothQuestion 4 During metaphase of mitosis:Question 4 options:DNA is replicatedSister chromatids line up together on the equatorDNA is uncoiled and in useSpindle fibers are absentPreparation for cell division beginsQuestion 5 During cell division, cytokinesis is completed by the end of:Question 5 options:prophaseanaphasemetaphasetelophaseinterphaseQuestion 6 Crossing over or recombination most frequently occurs during:Question 6 options:prophase I of meiosismetaphase II of meiosisprophase of mitosistelophase of mitosisprophase II of meiosisQuestion 7 In...
7. During meiosis II, Points: 1 @ sister, chrom sister chromatids of each chromosome are separated from each other. cytokinesis results in the formation of a total of two cells. (C) homologous chromosomes separate. sister chromatids exchange parts. E) homologous chromosomes pair up. 10 Shown below is a cell under going of meiosis. Points: 1 prophase II telophase telophase II prophase
SUMMARY – COMPARE MITOSIS AND MEIOSIS 1. What structures are present in the phases? How many? (Use numbers like 2n, 1n, etc., and terms like dyads, tetrads, replicated chromosomes, chromatids, chromosomes, ...) Phase Mitosis Meiosis I Meiosis II Prophase Metaphase Anaphase Telophase Cytokinesis Ploidy level after all divisions of 2n cell Number & type of cells produced by division(s) Function in life cycle
HILMIUSIS AND MEIOSIS 1. What structures are present in the phases? How many? (Use numbers like 2n, 1n, etc., and terms like dyads, tetrads, replicated chromosomes, chromatids, chromosomes,...) Mitosis Phase Prophase Meiosis Meiosis II Metaphase Anaphase Telophase Cytokinesis Ploidy level after all divisions of 2n cell Number & type of cells produced by division(s) Function in life cycle