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Which of the following is an accurate description of the probes used in DNA microarrays? Question...

Which of the following is an accurate description of the probes used in DNA microarrays?

Question 4 options:

Radiolabeled single stranded DNA fragments used to visualize complementary DNA bound to a solid surface

cDNA copies of fragmented RNA isolated from samples cells

Specific DNA fragments labeled with a fluorophore and bound in an ordered pattern to a microchip

Specific DNA fragments bound in an ordered pattern to a microchip

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Answer #1

Answer 4. Option 1, radiolabeled ssDNA fragments used to visualize..

In microarray, thousand of short strands of ssDNA are embedded as probes. These probes are manufactured artificially from the known genes. Each probe has a unique nucleotide sequence that is complementary to the target mRNA sequence.

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