Which of the following is an accurate description of the probes used in DNA microarrays?
Question 4 options:
Radiolabeled single stranded DNA fragments used to visualize complementary DNA bound to a solid surface |
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cDNA copies of fragmented RNA isolated from samples cells |
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Specific DNA fragments labeled with a fluorophore and bound in an ordered pattern to a microchip |
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Specific DNA fragments bound in an ordered pattern to a microchip |
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More than one of these responses is correct |
Answer 4. Option 1, radiolabeled ssDNA fragments used to visualize..
In microarray, thousand of short strands of ssDNA are embedded as probes. These probes are manufactured artificially from the known genes. Each probe has a unique nucleotide sequence that is complementary to the target mRNA sequence.
Which of the following is an accurate description of the probes used in DNA microarrays? Question...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
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table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...