You found a bacterium that has a genome of 2 millions base pairs. How long is the stretch of 16S rDNA that you want to sequence to make sure that you can know what specific species it is? You will first assume that there is no history in biology and then you will say in which direction the length of the DNA stretch (bigger or smaller) if you suppose that all bacterial species have evolved from a common ancestor.
With 16S there are some roles to identify your isolates :-
1-as much nucleotides you get from sequencing the better the identification it is. You can get this by sequencing both limits,or by fragments.
2-with 2 million base pairs it is sufficient to identify the genus level and sometimes the species level in case of non crowded genus.
3- in case of some crowded genus such as bacillus or streptomyces it is very difficult to get beyond the genus level (you just approach the species or some times so called complex or group) and in this case you say the most related species is__________or belong to the group of_________
4- for the stain level you should apply either sequencing of other genes or other biochemistry-immunology tests
Sequencing should be done from both sides(5' and 3' end) of template strand
After sequencing both sequence should paired ,their ends should merged in between ,otherwise PCR artifacts create problems, there will be a chimera problem.
For complete identification you should need long stretch of sequence (for 16S RNA size is 1500 base pairs).
You found a bacterium that has a genome of 2 millions base pairs. How long is...
You found a bacterium that has a genome of 2 millions base pairs. How long is the stretch of 16S rDNA that you want to sequence to make sure that you can know what specific species it is? You will first assume that there is no history in biology and then you will say in which direction the length of the DNA stretch (bigger or smaller) if you suppose that all bacterial species have evolved from a common ancestor. *
You PCR amplify a 500 bp (base pairs) piece of DNA that has
diagnostic value in determining whether a patient has a mutation
within a specific DNA region. You know that this DNA segment of the
“normal” gene does not include an EcoRI restriction site; but the
mutated DNA segment of the same gene contains an EcoRI restriction
site due to a point mutation at the 100th bp from the 5’ DNA end.
After PCR amplification, you subject your DNA...
A cell's genome is its blueprint for life. However, what is the bare minimum number of genes needed to sustain a free-living cell? This is a question that microbiologists at the J. Craig Venter Institute (JCVI) have attempted to answer ever since they sequenced the genomes of several Mycoplasma species in the 1990s. Because Mycoplasma species are parasitic bacteria, their genomes are already reduced in size and hence provide an excellent foundation for creating a "minimal cell." However, little did...
1 Overview and Background Many of the assignments in this course will introduce you to topics in computational biology. You do not need to know anything about biology to do these assignments other than what is contained in the description itself. The objective of each assignment is for you to acquire certain particular skills or knowledge, and the choice of topic is independent of that objective. Sometimes the topics will be related to computational problems in biology, chemistry, or physics,...
Please read the article bellow and discuss the shift in the
company's approach to genetic analysis. Please also discuss what
you think about personal genomic companies' approaches to research.
Feel free to compare 23andMe's polices on research with another
company's. Did you think the FDA was right in prohibiting 23andMe
from providing health information?
These are some sample talking points to get you thinking about
the ethics of genetic research in the context of Big Data. You
don't have to...
please Identify the key points and main thesis of the
article
2. Describe the skills you will need to develop to manage the
hospital of the future.
use critical analysis doing these questions
Suggestion for writing assignmemnt make believe the reader has
never read the article -what are the key points you would want the
reader to know in order to understand the hospital of the future.
In addition, managers, executives do not have time to read--so
again what key...
Please read the article and answer about questions. You and the Law Business and law are inseparable. For B-Money, the two predictably merged when he was negotiat- ing a deal for his tracks. At other times, the merger is unpredictable, like when your business faces an unexpected auto accident, product recall, or government regulation change. In either type of situation, when business owners know the law, they can better protect themselves and sometimes even avoid the problems completely. This chapter...
I need help with my very last assignment of this term
PLEASE!!, and here are the instructions: After reading Chapter Two,
“Keys to Successful IT Governance,” from Roger Kroft and Guy
Scalzi’s book entitled, IT Governance in Hospitals and Health
Systems, please refer to the following assignment instructions
below.
This chapter consists of interviews with executives
identifying mistakes that are made when governing healthcare
information technology (IT). The chapter is broken down into
subheadings listing areas of importance to understand...
need help with this ..
From the pages 571-587 attached below.
1a) Suppose a population of guppies was infected with a
parasite. In that population a mutation results in a parasite
resistant genotype that spreads through the population through
natural selection. A subsequent mutation in the parasite results in
a genotype that is unaffected by the newly evolved resistant guppy
genotype. What is the name of the hypothesis that explains this
host parasite “arms race”.?
1b) What is this name...