How would you determine the concentration of an unknown NiCl2 solution, if you have enough NiCl2...
How would you determine the concentration of an unknown NiCl2 solution, if you have enough NiCl2 as a powder, a Spectrovis Plus and all the necessary equipment? (Absorption maxima is observed at lamda = 610 nm)
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How would you determine the concentration of an unknown NiCl2
solution, if you have enough NiCl2...
need help with Concentration solutions Determine the Cu?molar concentration of each unknown solution. For unknown Cu?...
need help with Concentration solutions
Determine the Cu?molar concentration of each unknown solution. For unknown Cu? solution 1, the absorbance = 0.176 at 675 nm. Unknown Cul solution 1 concentration = _м For unknown Cu? solution 2, the absorbance =0.827 at 675 nm. Unknown Cu? solution 2 concentration - M Logger Profile Edit Experiment Data Analyze Insert Options Page Help Help Untitled AoQ 121/ O - Page 1 - No Device Connected Data Set ConcAbs (M 0.012 0.055 0.024 0.106...
6. Suppose your TA gives you a test tube containing a phosphate solution of unknown concentration...
6. Suppose your TA gives you a test tube containing a phosphate solution of unknown concentration and asks you to analyze it. Your TA is kind enough to give you a hint: the unknown solution has a phosphate concentration between 4.00 x 105 M and 8.00 x 103M. How would your sample preparation procedure for analyzing this unknown solution differ from the sample preparation procedure for the soda pop?
6. Suppose your TA gives you a test tube containing a...
14 CHAPTER 1 solution of Cu(NO), and a solution with an unknown concentration of about determine...
14 CHAPTER 1 solution of Cu(NO), and a solution with an unknown concentration of about determine the concentration of the unknown by measuring its absorbance and 2. A student has a 0.100 M solution of Cu(NO), and a soluti 0.00500 M. She wants to determine the concentration of the unik plotting it on a graph of absorbance against concentration. (a) List the equipment needed to prepare the solutions required for the graph. (b) State and explain the number of standard...
You need to neutralize a solution of an unknown concentration of sodium hydroxide before you can...
You need to neutralize a solution of an unknown concentration of sodium hydroxide before you can dispose of it. In the lab, you have a 5.0M bottle of hydrochloric acid, however, you want to dilute that first to use a less concentrated (and less dangerous) solution. So, you decide to use a 0.1M solution of hydrochloric acid solution and to make 2.0 litres of it. (a) Show all calculations for how you would do this. (b) Once you have your...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph below is the BSA standard curve. In the cuvette, you add 25 ul of the unknown solution X, 200 ul of the Bradford’s reagent and 775 ul dH2O. You obtain an absorbance of 0.58 at 595nm. Use all the information to calculate the concentration of the unknown solution X. Show work Y- 0.0457x R^2- 0.9901
An aspirin solution of unknown concentration was studied using ultraviolet spectroscopy. This solution had a percent...
An aspirin solution of unknown concentration was studied using ultraviolet spectroscopy. This solution had a percent transmittance of 37.3 when measured at 273 nm in a 1.000-cm cell. Aspirin is known to have a molar absorptivity of 741 M-1cm-1 at this wavelength. a. Calculate the absorbance of this solution b. What is the concentration of aspirin in this solution? c. What would be the absorbance of a solution prepared by taking 10.00mL of this solution and diluting to 100.00mL in...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5 mg/mL. After you add 2 mL of your unknown to 8 mL of buffer, you obtain an absorption spectrum of your colored compound. You record an Absorbance value of 0.57 at its lambda max, 460 nm. The light path is 1 cm. Calculate the molar concentration (M) of your diluted sample. Show your work. B. Calculate the extinction coefficient (M-1cm-1) of your diluted...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5 mg/mL. After you add 2 mL of your unknown to 8 mL of buffer, you obtain an absorption spectrum of your colored compound. You record an Absorbance value of 0.57 at its lambda max, 460 nm. The light path is 1 cm. Calculate the molar concentration (M) of your diluted sample. Show your work. B. Calculate the extinction coefficient (M-1cm-1) of your diluted...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
In lab, you diluted your unknown protein solution by combining 14 ?L of unknown protein solution...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
need help with Concentration solutions
Determine the Cu?molar concentration of each unknown solution. For unknown Cu? solution 1, the absorbance = 0.176 at 675 nm. Unknown Cul solution 1 concentration = _м For unknown Cu? solution 2, the absorbance =0.827 at 675 nm. Unknown Cu? solution 2 concentration - M Logger Profile Edit Experiment Data Analyze Insert Options Page Help Help Untitled AoQ 121/ O - Page 1 - No Device Connected Data Set ConcAbs (M 0.012 0.055 0.024 0.106...
6. Suppose your TA gives you a test tube containing a phosphate solution of unknown concentration and asks you to analyze it. Your TA is kind enough to give you a hint: the unknown solution has a phosphate concentration between 4.00 x 105 M and 8.00 x 103M. How would your sample preparation procedure for analyzing this unknown solution differ from the sample preparation procedure for the soda pop?
6. Suppose your TA gives you a test tube containing a...
14 CHAPTER 1 solution of Cu(NO), and a solution with an unknown concentration of about determine the concentration of the unknown by measuring its absorbance and 2. A student has a 0.100 M solution of Cu(NO), and a soluti 0.00500 M. She wants to determine the concentration of the unik plotting it on a graph of absorbance against concentration. (a) List the equipment needed to prepare the solutions required for the graph. (b) State and explain the number of standard...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
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