Question

You want to track translation of your transcription factor within a pancreatic cell for your Excellence in Research project. You perform a pulse-chase experiment, where radioactive amino acids are introduced to the cell over a short period of time (pulse) and then are washed away (chase). The radioactive amino acids will be introduced into newly synthesized protein and you can track the radioactive proteins as they are sorted throughout the cell. You also add a drug that prevents translation of all proteins except your transcription factor. Where would the first radioactive signals appear after you wash away the free radioactive amino acids?

1. The Golgi

2. The cytoplasm

3. Both the ER and the cytoplasm

4. The nucleus

5. The ER

Answer 1

radioactive amino acids are given, also a drug that inhibits translation of proteins other than transcription factor are also given so only transcription factors will incorporate radioactive amino acids transcription factors localize to the nucleus to carry out the transcription of genes so when free radioactive amino acids are washed away signal will come from nucleus only, so the answer is 4) the nucleus