10. Describe and diagram the mechanism of regulating gene expression utilized for the E. coli trp...
10. Describe and diagram the mechanism of regulating gene expression utilized for the E. coli trp operon. Compare and contrast this to the general strategy utilized for the lac operon.
Homework Answers
Tryptophan or trp operon in E. coli is operated fro synthesis of tryptophan from chorismate, where five structural genes (Gene A to E in recerse order) are involved. This operon is regulated by two mechanisms: (1) negatively regulated by tryptophan via repressor binding (2) attenuation mechanism.
The complete diagram of the genetic map for tryptophan operon is given below:
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10. Describe and diagram the mechanism of regulating gene
expression utilized for the E. coli trp...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of lac operon in E.coli. b. Role of CAP and cAMP the activator binding site c. Role of Lac Repressor and Operator and Inducer d. Role of Looping e. Effect of mutations f. Recessive v dominant mutations g. Complementing v noncomplementing mutations. Describe the regulation of the trp operon in E. coli. Describe at least three kinds of molecular switches involved in gene regulation. What...
Answer please E. coli expresses a specific gene in the presence of lactose. The expression of...
Answer please
E. coli expresses a specific gene in the presence of lactose. The expression of this gene is under control by the lac operon, a sequence of nucleotides in the DNA which control the lac Operon al DNA ) laci CAP) (o) laczlacY
1) Describe the similarities and differences between the lac operon in E. coli and the GAL...
1) Describe the similarities and differences between the lac operon in E. coli and the GAL system in yeast. 2) What effect does mutation at nucleotides within a silencer element have on the expression of the gene associated with the silencer? Why does it have this effect?
1. If you isolated a mutation that disrupted a positive regulatory gene for the thr operon...
1. If you isolated a mutation that disrupted a positive regulatory gene for the thr operon (which contains the genes needed to synthesize the amino acid threonine), what would the phenotype be? 2. Compare and contrast the positive regulation of expression of the lac operon by the cAMP-CAP complex with the negative regulation of expression of the lac operon by the LacI protein. You should have several similarities and parallel differences. 3. All human beings have a rich growth of...
In the context of E. coli fepCDG operon gene expression, Fe is A. an inducer. B....
In the context of E. coli fepCDG operon gene expression, Fe is A. an inducer. B. a corepressor. C. an effector. D. Two of the above.
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of this positive regulation of the lac operon: What is the activator? Would it work in cis or trans? What molecule binds to the allosteric domain of the activator? What influences strongly the levels of the molecule you answered for part b? To what regulatory sequence does the activator bind? What part/domain of the activator? What is the effect of this binding? Name the two...
Trp Operon 5. The rate of transcription of the trp operon in E. coli is controlled by both repression and attenuation dr papde ia) Alternate secondary structures formed by the trpl tranecript Alterna...
Trp Operon 5. The rate of transcription of the trp operon in E. coli is controlled by both repression and attenuation dr papde ia) Alternate secondary structures formed by the trpl tranecript Alternate 2 Regions 2 and 3 Atemate 1: Regions 1 and 2 basepared and regions 3 and 4 basepsired 54 140 Stop codon Transcribtion termination hairpin can NOT form a) Diagram and explain repression and attenuation regulatory mechanisms for the trp operon when tryptophan is present and absent....
If lactose is accumulating outside of E. coli and is unable to enter, which structural gene...
If lactose is accumulating outside of E. coli and is unable to enter, which structural gene of the lac operon is most likely mutated? (d) Select one: a. lacY b. lacI c. lacA d. lacZ e. lacX
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
Answer please
E. coli expresses a specific gene in the presence of lactose. The expression of this gene is under control by the lac operon, a sequence of nucleotides in the DNA which control the lac Operon al DNA ) laci CAP) (o) laczlacY
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Trp Operon 5. The rate of transcription of the trp operon in E. coli is controlled by both repression and attenuation dr papde ia) Alternate secondary structures formed by the trpl tranecript Alternate 2 Regions 2 and 3 Atemate 1: Regions 1 and 2 basepared and regions 3 and 4 basepsired 54 140 Stop codon Transcribtion termination hairpin can NOT form a) Diagram and explain repression and attenuation regulatory mechanisms for the trp operon when tryptophan is present and absent....
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
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