we transform bacteria to make medically useful human proteins such as insulin and growth hormone. explain...
Homework Answers
We basically make a plasmid molecule and insert our gene which codes for our protein of interest that maybe insulin and then transform it into the ecoli bacteria and culture these bacterias to as they are multiplied in large quantities and very easily in ashort period of time our gene of interst also gets multiplied and many copies are generated which can then be isolated for different applications.Large quantities can be provide by providing favourable conditions and nutrients for the growth of the E coli molecules.
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transform bacteria to make medically useful human proteins such as
insulin and growth hormone. explain...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Only information given Explain how you might engineer E. coli to produce human growth hormone (HGH)...
Only information given
Explain how you might engineer E. coli to produce human growth hormone (HGH) using the following. E. coli containing a plasmid DNA carrying the gene for HGH DNAIigase A restriction enzyme Equipment for manipulating and growing bacteria A method for extracting and purifying the hormone An appropriate DNA probe For this problem, assume the HGH gene lacks introns.
35. Insulin is a peptide (protein) hormone secreted by B-cells located in the pancreas. To be...
35. Insulin is a peptide (protein) hormone secreted by B-cells located in the pancreas. To be protein) hormone used to regulate the level of glucose in the bloodstream. It is produced and performed. cated in the pancreas. To better understand how insulin is secreted, the following experiments were a. The mature me mature mRNA for insulin encodes a protein that is 110 amino acids in length. If the genes t is 110 amino acids in length. If the gene is...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity chromatography but also to ___________. A. overexpress the proteins in bacteria B. to reduce toxicity of the foreign proteins C. for proper folding and keeping the proteins soluble D. to trace the cytological location of the foreign proteins 15. You have constructed an insulin-GST fusion protein and expressed it in E. coli. You want to separate the recombinant...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
DATE: NURS 208A NAME: 1. The function of ribosomes is to: 6. Inb Digest foreign bacteria...
DATE: NURS 208A NAME: 1. The function of ribosomes is to: 6. Inb Digest foreign bacteria and toxic substances Produces energy from glucose y Aid in cell division Synthesize proteins Which of the following statement is false about the cell membrane? 2. Cell membrane is composed of phospholipid layer The cell membrane prefers large molecules to pass through freely Cell membrane is a semipermeable boundary which determines what can enter and ex the cell The exterior surface is hydrophilic phosphate...
Food Microbiology Purpose To utilize the process of fermentation to make yogurt To describe organisms responsible...
Food Microbiology Purpose To utilize the process of fermentation to make yogurt To describe organisms responsible for food borne illnesses and summarize a recent outbreak Introduction Microorganisms have been used for centuries for food preservation and to improve or change its taste. Evidence exists that yogurt, which is milk fermented by bacteria, has been around for over 4000 years. Today, many of the foods we eat are the result of microorganisms acting on foods for a specific and desired effect....
Please answer ALL QUESTIONS. If your not going to answer them please leave it for someone...
Please answer ALL QUESTIONS. If your not going to answer them
please leave it for someone else to answer.
35) A person is rushed to the Emergency Room of a ch a n c e treatment with a drug called Atropine Atropinis asympathom , which i s the effects of the the nervous system. What symptomed this point have that wanted the wse of Bir A) a wound that would not det C) wery Nigh blood pressure D) a depressed...
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Only information given
Explain how you might engineer E. coli to produce human growth hormone (HGH) using the following. E. coli containing a plasmid DNA carrying the gene for HGH DNAIigase A restriction enzyme Equipment for manipulating and growing bacteria A method for extracting and purifying the hormone An appropriate DNA probe For this problem, assume the HGH gene lacks introns.
35. Insulin is a peptide (protein) hormone secreted by B-cells located in the pancreas. To be protein) hormone used to regulate the level of glucose in the bloodstream. It is produced and performed. cated in the pancreas. To better understand how insulin is secreted, the following experiments were a. The mature me mature mRNA for insulin encodes a protein that is 110 amino acids in length. If the genes t is 110 amino acids in length. If the gene is...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
DATE: NURS 208A NAME: 1. The function of ribosomes is to: 6. Inb Digest foreign bacteria and toxic substances Produces energy from glucose y Aid in cell division Synthesize proteins Which of the following statement is false about the cell membrane? 2. Cell membrane is composed of phospholipid layer The cell membrane prefers large molecules to pass through freely Cell membrane is a semipermeable boundary which determines what can enter and ex the cell The exterior surface is hydrophilic phosphate...
Please answer ALL QUESTIONS. If your not going to answer them
please leave it for someone else to answer.
35) A person is rushed to the Emergency Room of a ch a n c e treatment with a drug called Atropine Atropinis asympathom , which i s the effects of the the nervous system. What symptomed this point have that wanted the wse of Bir A) a wound that would not det C) wery Nigh blood pressure D) a depressed...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
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