If you start with 47 DNA segments, how many total copies of DNA will you have...
If you start with 47 DNA segments, how many total copies of DNA will you have after 12 cycles of PCR?
Also what does attenuated mean in regards to the K12 strain of E. coli being used in lab? Is the answer “we have removed the gene that allows the bacteria to replicate”?
Thank you!!
Homework Answers
The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA.
An attenuated virus is a weakened, less vigorous virus. A vaccine against a viral disease can be made from an attenuated, less virulent strain of the virus, a virus capable of stimulating an immune response and creating immunity but not causing illness.Basically, the attenuated virus administered is unable to replicate sufficiently to cause disease in the recipient, but will still induce an immune response that can protect against infection before the virus is cleared by the immune system.
Please do give thumbs up ?
Add Answer to:
If you start
with 47 DNA segments, how many total copies of DNA will you have...
Suppose you start a PCR reaction with 3 copies of a double stranded DNA fragment. How...
Suppose you start a PCR reaction with 3 copies of a double stranded DNA fragment. How many copies will be present after 4 replication cycles? a. 7 b. 64 c. 48 d. 24 e. 8
if you start with 2 pieces of DNA, after 4 cycles of PCR how many would...
if you start with 2 pieces of DNA, after 4 cycles of PCR how many would you have?
You determine that you have only three copies left of an important DNA fragment, so you...
You determine that you have only three copies left of an important DNA fragment, so you decide to amplify it. Using flanking primers, how many PCR cycles would you have to run to generate over one billion (109) copies of the fragment? I know the answer is 29. I just don't understand how to get that answer.
8. Explain how the LacZ gene is used in recombinant DNA technology. 9. You LOVE spinach....
8. Explain how the LacZ gene is used in recombinant DNA technology. 9. You LOVE spinach. This morning you heard on the news that E.coli O157:H7 has contaminated 90% of the spinach crops in your area. E.coli O157://7 is a pathogenic strain of bacteria that has acquired a gene that produces a toxin that gives people hemorrhagic diarrhea. You are bummed because you CAN'T live without your spinach! You recently began working in a lab that has a PCR machine...
Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This...
Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This is a lab we did and some homework questions regarding the sequencing rDNA to find unknown bacteria. I hope by answering these I can have better understanding of the process. The more descriptive the better. Thank you! 1. A. Name the gene that we will be sequencing in order to identify the unknown. Explain why this region is considered to be the target for...
Assuming that you start with one template DNA molecule, how many total DNA molecules would you...
Assuming that you start with one template DNA molecule, how many total DNA molecules would you have after 30 cycles? O 6.48 x 105 060 0 1,073 O ~1.07 x 109 Submit Answer Tries 0/99
not sure what the correct answers are To be export-ready, an mRNA molecule must: have its...
not sure what the correct answers are
To be export-ready, an mRNA molecule must: have its 5' cap removed be bound by EJCS have its poly-A tail removed have at least half of its introns removed od 80e covou The extension temperature used during PCR is determined by: the length of the PCR product you want to generate the DNA polymerase that is being used the annealing temperature of your primers x the source of your template DNA (eg, genomic...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
4. p53 is an important cancer suppressor gene. To study its function, it is very useful to have p53 knock-out mice. How can one best prepare a strain of p53 knock-out mice? Please start with the optim...
4. p53 is an important cancer suppressor gene. To study its function, it is very useful to have p53 knock-out mice. How can one best prepare a strain of p53 knock-out mice? Please start with the optimal method to introduce mutations into DNA, and then describe how you would incorporate such DNA into the mouse genome. List all major steps. please give detailed answer, thank you!
8. Explain how the LacZ gene is used in recombinant DNA technology. 9. You LOVE spinach. This morning you heard on the news that E.coli O157:H7 has contaminated 90% of the spinach crops in your area. E.coli O157://7 is a pathogenic strain of bacteria that has acquired a gene that produces a toxin that gives people hemorrhagic diarrhea. You are bummed because you CAN'T live without your spinach! You recently began working in a lab that has a PCR machine...
Assuming that you start with one template DNA molecule, how many total DNA molecules would you have after 30 cycles? O 6.48 x 105 060 0 1,073 O ~1.07 x 109 Submit Answer Tries 0/99
not sure what the correct answers are
To be export-ready, an mRNA molecule must: have its 5' cap removed be bound by EJCS have its poly-A tail removed have at least half of its introns removed od 80e covou The extension temperature used during PCR is determined by: the length of the PCR product you want to generate the DNA polymerase that is being used the annealing temperature of your primers x the source of your template DNA (eg, genomic...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
Most questions answered within 3 hours.
-
Determine the temperature (in Celsius) at which 1.00 mole of an
ideal gas will have a...
asked 14 minutes ago -
Japan’s combination of X and Y
Canada’s combination of X and Y
100x and 0y
50x...
asked 6 minutes ago -
[1] Household statistics include individuals living alone or in
groups in:
A) apartments.
B) military barracks....
asked 17 minutes ago -
What is the % w/v when 80 mL of a 2.0% solution is mixed with 50...
asked 20 minutes ago -
How can I solve the following using a TI83
Claim: Most adults would erase all of...
asked 33 minutes ago -
Analysis of 3-ethyl-3-buten-2-ol gave C, 72.13%; H, 11.92%.
Calculate the percent deviation of these results from...
asked 30 minutes ago -
Which VALS segment is most likely to have a top of the line
brand new (2015)...
asked 34 minutes ago -
Write a program to score the paper-rock-scissor game. Each of
two users types in either P,R...
asked 54 minutes ago -
Calculate the equillibrium constent K for a redox reaction that
has E°cell = -.98 V at...
asked 1 hour ago -
A concave spherical mirror has a radius of curvature of
magnitude 19.6 cm.
(a) Find the...
asked 1 hour ago -
3. draw a diagram of the magnetic field:
a. around a long straight wire with a...
asked 1 hour ago -
If you titrated 30.0 mL of 0.1 M HCl with 0.1 M NaOH, indicate
the approximate...
asked 1 hour ago