Question

8. Explain how the LacZ gene is used in recombinant DNA technology. 9. You LOVE spinach. This morning you heard on the news that E.coli O157:H7 has contaminated 90% of the spinach crops in your area. E.coli O157://7 is a pathogenic strain of bacteria that has acquired a gene that produces a toxin that gives people hemorrhagic diarrhea. You are bummed because you CAN'T live without your spinach! You recently began working in a lab that has a PCR machine that you can use. Explain how you could use PCR to determine if the spinach you have is infected with E.coli O157:H7 or not. (3 pts)

Answer 1

NACHALY os Treto other TE nologя фоcеs g Lacz gene called B It is a sure sidase an enze and galactose substrale x g ode for B-galacto Poresence of an This is also gene used in crecombinant technology apo led Blue - white Screening Powocess. a Sorenable marker gene that encode for an enzyme that splits lactose into glucose galactose , B galactosidase act on colorless sale x gal and develop Blue color, so nce of dach gene in system can be assayea in boesence of xgal s is also known as B - galaclosidase gene Complementation. . certain deletion mutation in lac z gene crescelt in formation of protein that lack some N-terminal residue which pucevent homotellamer formation 9n such mutant subunit assembly and enzyme activity restored by the presence of small flagment called a - peptide, vector contain x-peptide. Those produce blue color means functional Detaylactosidase present no insertional inactivation occur 9 So vector is not precombinant vector no new DNA insected in vector