Question

mutations Troll questull alcului Iluuuu TUM 3. You are studying a genetic disease, and hypothesize that mutations in a specific gene cause this disease, so you sequence the gene from various people with and without the disease. Below are a sequencing gel and automated sequencing chromatograph for someone carrying the normal gene. How would you expect each of the following mutations to change the appearance of a gel or the chromatograph? TGCA GAT insertions! everythi the righ a. A homozygous substitution (for example, changing the first T to an A) b. A heterozygous substitution C. A homozygous deletion or insertion d. A heterozygous deletion or insertion

Answer 1

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3. T G C A. (а) - АСТАССТА ARA A homozygous stebstitution of firest TAGA I G C A. 6 ТАТСТАС СТА A substitution of G with I Cheterozygores) (fircel G) - с го - G C Am ДG TAc cТА — Homozygoces deletion (deletion offerta G1 GүСАтет Ac cТА Heterozygotes insertion (insertion of GFC Bafter forest G)