You are studying a disease that is known to be caused by a single nucleotide change...
You are studying a disease that is known to be caused by a single nucleotide change in a single gene, although the effect this change ultimately has on the protein's structure and function is unknown. You have DNA samples from multiple patients that you suspect of having this disease. What is the most efficient way to test the samples for the relevant mutation?
Select one:
a. PCR amplification followed by Sanger DNA sequencing
b. PCR amplification followed by gel electrophoresis
c. High-throughput exome sequencing
d. High-throughput genome sequencing
e. Preimplantation genetic diagnosis
Homework Answers
a. PCR amplification followed by Sanger DNA sequencing
Sanger sequencing is a very effective tool to determine the sequence of a small DNA molecule. Since the mutation causing the disease is known, it would be possible to identify individuals who have the disease by amplifying DNA and then sequencing the DNA to determine which individuals have the disease.
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You are studying a disease that is known to be caused by a
single nucleotide change...
find three errors in the pragraph and reconstruct the gel DI Succuleluujective vi, a 16 points)...
find three errors in the pragraph and reconstruct the gel
DI Succuleluujective vi, a 16 points) PCR is a powerful tool used to isolate and make large quantities of a defined DNA fragment from a complex genome. PCR takes advantage of electrophoresis and synthetic oligonucleotides. Its starting material can be as small as a single cell. Because amplification is exponential, a single DNA molecule can be copied into hundreds of copies in a single day. Once a reference DNA sequence...
Suppose there is a genetic disorder caused by a single base change in a gene on...
Suppose there is a genetic disorder caused by a single base change in a gene on chromosome 11, which changes a cellular protein so that its function is altered. This mutation happens also to eliminate a cleavage site for a restriction endonuclease EcoRI (sequence: 5'-GAATTC-3'). If you isolated a portion of chromosome 11 DNA containing this defective gene, explain exactly how could you use restriction enzymes and gel electrophoresis to detect the base change. Please write 500 words or fewer.
mutations Troll questull alcului Iluuuu TUM 3. You are studying a genetic disease, and hypothesize that...
mutations Troll questull alcului Iluuuu TUM 3. You are studying a genetic disease, and hypothesize that mutations in a specific gene cause this disease, so you sequence the gene from various people with and without the disease. Below are a sequencing gel and automated sequencing chromatograph for someone carrying the normal gene. How would you expect each of the following mutations to change the appearance of a gel or the chromatograph? TGCA GAT insertions! everythi the righ a. A homozygous...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS WELL. THANKSSSSSSS 1. You want...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
Can someone help me with this homework question on gel electrophoresis? Translocation and deletion 3. You...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein...
Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein gel electrophoresis. Protein gel electrophoresis different composition. ) is carried out in the same manner as DNA gel electrophoresis (see Fig. 10.8) except the gel hasa 1. Sickle-cell hemoglobin (Hb) migrates more Figure 10.10 Gel slowly toward the positive pole than normal hemoglobin (Hb) because the amino acid valine has no polar R groups, whereas the amino acid +Pole glutamic acid does have a...
You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in...
You PCR amplify a 500 bp (base pairs) piece of DNA that has
diagnostic value in determining whether a patient has a mutation
within a specific DNA region. You know that this DNA segment of the
“normal” gene does not include an EcoRI restriction site; but the
mutated DNA segment of the same gene contains an EcoRI restriction
site due to a point mutation at the 100th bp from the 5’ DNA end.
After PCR amplification, you subject your DNA...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
Paragraph Styles The technological approaches applied for ancient DNA study can also be used for forensic...
Paragraph Styles The technological approaches applied for ancient DNA study can also be used for forensic genetic analysis in difficult cases where only microscopic amounts of material are available or the DNA has been severely damaged. Some of these approaches were used in the genetic expertise of the putative remains of the family of the last Russian Emperor Nicholas II Romanov. There is historical evidence that Prince Alexey suffered from severe bleeding that is characteristic of hemophilia. It is now...
The technological approaches applied for ancient cal approaches applied for ancient DNA study can also be...
The technological approaches applied for ancient cal approaches applied for ancient DNA study can also be used for forensic genetic analysis in Where only microscopic amounts of material are available or the DNA has been severely damaged. Some of these approaches were used in the genetic expertise of the putative remains on the last Russian Emperor Nicholas II Romanov. is historical evidence that Prince Alexey suffered from severe bleeding that is characteristic of philia. It is now known that hemophilia...
find three errors in the pragraph and reconstruct the gel
DI Succuleluujective vi, a 16 points) PCR is a powerful tool used to isolate and make large quantities of a defined DNA fragment from a complex genome. PCR takes advantage of electrophoresis and synthetic oligonucleotides. Its starting material can be as small as a single cell. Because amplification is exponential, a single DNA molecule can be copied into hundreds of copies in a single day. Once a reference DNA sequence...
mutations Troll questull alcului Iluuuu TUM 3. You are studying a genetic disease, and hypothesize that mutations in a specific gene cause this disease, so you sequence the gene from various people with and without the disease. Below are a sequencing gel and automated sequencing chromatograph for someone carrying the normal gene. How would you expect each of the following mutations to change the appearance of a gel or the chromatograph? TGCA GAT insertions! everythi the righ a. A homozygous...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein gel electrophoresis. Protein gel electrophoresis different composition. ) is carried out in the same manner as DNA gel electrophoresis (see Fig. 10.8) except the gel hasa 1. Sickle-cell hemoglobin (Hb) migrates more Figure 10.10 Gel slowly toward the positive pole than normal hemoglobin (Hb) because the amino acid valine has no polar R groups, whereas the amino acid +Pole glutamic acid does have a...
You PCR amplify a 500 bp (base pairs) piece of DNA that has
diagnostic value in determining whether a patient has a mutation
within a specific DNA region. You know that this DNA segment of the
“normal” gene does not include an EcoRI restriction site; but the
mutated DNA segment of the same gene contains an EcoRI restriction
site due to a point mutation at the 100th bp from the 5’ DNA end.
After PCR amplification, you subject your DNA...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
Paragraph Styles The technological approaches applied for ancient DNA study can also be used for forensic genetic analysis in difficult cases where only microscopic amounts of material are available or the DNA has been severely damaged. Some of these approaches were used in the genetic expertise of the putative remains of the family of the last Russian Emperor Nicholas II Romanov. There is historical evidence that Prince Alexey suffered from severe bleeding that is characteristic of hemophilia. It is now...
The technological approaches applied for ancient cal approaches applied for ancient DNA study can also be used for forensic genetic analysis in Where only microscopic amounts of material are available or the DNA has been severely damaged. Some of these approaches were used in the genetic expertise of the putative remains on the last Russian Emperor Nicholas II Romanov. is historical evidence that Prince Alexey suffered from severe bleeding that is characteristic of philia. It is now known that hemophilia...
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